Ohkoshi S, Kato N, Kinoshita T, Hijikata M, Ohtsuyama Y, Okazaki N, Ohkura H, Hirohashi S, Honma A, Ozaki T
Virology Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1990 Sep;81(9):862-5. doi: 10.1111/j.1349-7006.1990.tb02658.x.
Sera obtained from patients with non-A, non-B hepatitis were examined for the presence of hepatitis C virus (HCV) genome by using the reverse transcription-polymerase chain reaction assay, as well as for antibody to HCV (anti-HCV) by using an enzyme-linked immunosorbent assay (ELISA). We also examined the presence of HCV RNA in liver tissue obtained by surgical resection of hepatocellular carcinoma. Among 33 patients, HCV RNA was detectable in 21 (64%), and the antibody was also positive in 21 (64%). Eighteen (55%) patients were positive for both assays. The two assays gave inconsistent results in 3 patients who were positive for HCV RNA but negative for anti-HCV, and in 3 other patients who were negative for HCV RNA and positive for anti-HCV. HCV RNA was also detected in 6 out of 10 non-cancerous liver tissue specimens and in 3 out of 7 tumor tissue specimens. Using the polymerase chain reaction, the HCV genome was detected directly in many specimens obtained from patients with non-A, non-B hepatitis, suggesting the presence of replicating virus in patients positive for anti-HCV. In addition, the differing results of the two assay systems suggest that the application of both is important for evaluation of the status of HCV infection.
采用逆转录-聚合酶链反应分析法检测非甲非乙型肝炎患者血清中丙型肝炎病毒（HCV）基因组的存在情况，并采用酶联免疫吸附测定法（ELISA）检测抗HCV抗体。我们还检测了通过手术切除肝细胞癌获得的肝组织中HCV RNA的存在情况。在33例患者中，21例（64%）可检测到HCV RNA，21例（64%）抗体也呈阳性。18例（55%）患者两种检测均为阳性。两种检测方法在3例HCV RNA阳性但抗HCV阴性的患者以及另外3例HCV RNA阴性但抗HCV阳性的患者中得出了不一致的结果。在10份非癌性肝组织标本中的6份以及7份肿瘤组织标本中的3份中也检测到了HCV RNA。使用聚合酶链反应，在许多非甲非乙型肝炎患者的标本中直接检测到了HCV基因组，这表明抗HCV阳性患者中存在正在复制的病毒。此外，两种检测系统结果的差异表明，同时应用这两种方法对于评估HCV感染状况很重要。
