Schrumpf E, Elgjo K, Fausa O, Haukenes G, Kvale D, Rollag H
Dept. of Medicine A, Rikshospitalet, Oslo, Norway.
Scand J Gastroenterol. 1990 Nov;25(11):1169-74. doi: 10.3109/00365529008998550.
The frequency of hepatitic C virus (HCV) antibodies was determined in two different laboratories in stored sera from 128 consecutive patients with chronic liver disease and from 41 healthy blood donors. Repeated measurements were performed in most patients. At the first determination the frequency of HCV antibodies was 7% in primary sclerosing cholangitis, 42% in primary biliary cirrhosis, 40% in autoimmune chronic active hepatitis, and 27% in alcoholic liver disease. The reproducibility of the determinations was rather poor, with a within-assay variation of 9.9%, whereas the between-assay variation was 34% and 47% in the two laboratories. There was a significant difference in the results obtained in the controls, depending on the handling of the sera. Freezing and thawing and, possibly, protracted storing of sera had a major impact on the assay and may have invalidated the results obtained in many studies. A significant association between IgG levels and titers of HCV antibodies was found in the total group of patients (p less than 0.005), in autoimmune chronic active hepatitis (p less than 0.005), and in primary biliary cirrhosis (p less than 0.01). It may be questioned whether the assay really is specific for anti-HCV antibodies in these patients. Whether HCV has anything to do with the etiology and pathogenesis of chronic liver disease apart from NANB-hepatitis is still undetermined.
在两个不同实验室中，对128例连续慢性肝病患者和41例健康献血者的储存血清检测丙型肝炎病毒（HCV）抗体频率。多数患者进行了重复检测。首次检测时，原发性硬化性胆管炎患者中HCV抗体频率为7%，原发性胆汁性肝硬化患者中为42%，自身免疫性慢性活动性肝炎患者中为40%，酒精性肝病患者中为27%。检测的重复性相当差，批内变异为9.9%，而两个实验室的批间变异分别为34%和47%。根据血清处理方式不同，对照组的检测结果存在显著差异。血清的冻融以及可能的长期储存对检测有重大影响，可能使许多研究结果无效。在全部患者组（p<0.005）、自身免疫性慢性活动性肝炎患者（p<0.005）和原发性胆汁性肝硬化患者（p<0.01）中，发现IgG水平与HCV抗体滴度之间存在显著关联。这些患者中该检测是否真的针对抗HCV抗体值得怀疑。除了非甲非乙型肝炎外，HCV与慢性肝病的病因和发病机制是否有关仍未确定。
