de Kozak Y, Mirshahi M, Boucheix C, Faure J P
Unité de Recherche d'Ophtalmologie, INSERM U 86-CNRS, Centre de Recherche des Cordeliers, Paris, France.
Reg Immunol. 1992 May-Jun;4(3):168-74.
Experimental autoimmune uveoretinitis (EAU) was induced in rnu/+ rats by one injection of retinal S-antigen (S-Ag) in complete Freund's adjuvant. Immunization of rats, before the S-Ag challenge, with a polyclonal antibody directed at the monoclonal antibody (mAb) S2D2, prevented the development of EAU in rats at clinical and histological levels. Lymph node cells from rats hyperimmunized with this anti-idiotype (anti-Id) S2D2 antibody were stimulated in vitro by anti-Id S2D2, S-Ag, and mAb S2D2. These findings suggest that anti-S2D2 antibody acts as an internal image at the T-cell level for the epitope of S-Ag recognized by mAb S2D2 (epitope S2). From these results, it appears that cells bearing receptors for the internal image of the epitope S2, which is not pathogenic when injected emulsified in adjuvants, are able to inhibit S-Ag-induced EAU.
通过在完全弗氏佐剂中单次注射视网膜 S 抗原（S-Ag），在 rnu/+大鼠中诱导实验性自身免疫性葡萄膜视网膜炎（EAU）。在用 S-Ag 攻击之前，用针对单克隆抗体（mAb）S2D2 的多克隆抗体对大鼠进行免疫，可在临床和组织学水平上预防大鼠 EAU 的发生。用这种抗独特型（抗-Id）S2D2 抗体进行超免疫的大鼠的淋巴结细胞，在体外被抗-Id S2D2、S-Ag 和 mAb S2D2 刺激。这些发现表明，抗 S2D2 抗体在 T 细胞水平上作为 mAb S2D2 识别的 S-Ag 表位（表位 S2）的内影像起作用。从这些结果来看，携带表位 S2 内影像受体的细胞，当以佐剂乳化形式注射时无致病性，却能够抑制 S-Ag 诱导的 EAU。
