De Kozak Y, Mirshahi M, Boucheix C, Faure J P
Eur J Immunol. 1985 Nov;15(11):1107-11. doi: 10.1002/eji.1830151108.
Mouse monoclonal antibodies (mAb), of either IgG2a or IgG2b isotypes, specific for the retinal S-autoantigen (S-Ag) or a pool of rat anti-S-Ag sera prevented experimental autoimmune uveoretinitis in Lewis rats when injected i.p. at the time of immunization. Control mAb of the same isotypes, irrelevant to S-Ag, had no inhibitory effect. The humoral response to S-Ag, as studied by enzyme-linked immunosorbent assay using a mouse mAb specific for rat kappa chain, was moderately but significantly reduced in suppressed animals. The rapid disappearance of the injected mAb from rat sera, as measured using a rat mAb specific for mouse kappa chain, could be explained by its complexing with either autologous antigen released from the retina at the site of inflammation, or anti-idiotypic antibodies.
IgG2a或IgG2b同种型的小鼠单克隆抗体（mAb），对视网膜S自身抗原（S-Ag）具有特异性，或者一组大鼠抗S-Ag血清，在免疫时腹腔注射可预防Lewis大鼠的实验性自身免疫性葡萄膜视网膜炎。相同同种型的对照mAb，与S-Ag无关，没有抑制作用。使用对大鼠κ链具有特异性的小鼠mAb通过酶联免疫吸附测定研究，对S-Ag的体液反应在受抑制的动物中适度但显著降低。使用对小鼠κ链具有特异性的大鼠mAb测量，注射的mAb从大鼠血清中快速消失，这可以通过其与炎症部位从视网膜释放的自体抗原或抗独特型抗体结合来解释。
