犬全身性红斑狼疮。II：抗核抗体。

Canine systemic lupus erythematosus. II: Antinuclear antibodies.

作者信息

Monier J C, Ritter J, Caux C, Chabanne L, Fournel C, Venet C, Rigal D

机构信息

Laboratoire d'Immunologie, UFR de Médecine A. Carrel, Lyon, France.

出版信息

Lupus. 1992 Oct;1(5):287-93.

PMID:1284631

Abstract

The frequency and the specificities of antinuclear antibodies (ANAb) were studied in dogs with systemic lupus erythematosus (SLE) and compared to those found in normal dogs and in dogs with various infectious diseases. Whole ANAb were detected by immunofluorescence. Anti-double-stranded DNA Ab were found in only 2% of SLE dogs, whereas anti-single-stranded DNA Ab were present in 21.4% of SLE dogs and in 26.8% of dogs with infectious disease. Antihistone Ab were frequently observed in SLE dogs (71%) and are essentially directed against trypsin-resistant epitopes of H3, H4 and H2A. The Western blots of nuclear extracts of HeLa cells were recognized mainly by type 1 Ab (30%, reacting with bands of 43, 36, 35, 34, 30 and 27 kDa) and by anti-Sm Ab (12%) associated with anti-RNP Ab. Anti-SSA and anti-SSB Ab were rare.

摘要

研究了系统性红斑狼疮（SLE）犬的抗核抗体（ANAb）频率和特异性，并与正常犬及患有各种传染病的犬进行比较。通过免疫荧光检测全抗核抗体。仅2%的SLE犬发现抗双链DNA抗体，而21.4%的SLE犬及26.8%的传染病犬存在抗单链DNA抗体。抗组蛋白抗体在SLE犬中经常观察到（71%），且主要针对H3、H4和H2A的抗胰蛋白酶表位。HeLa细胞核提取物的蛋白质免疫印迹主要被1型抗体（30%，与43、36、35、34、30和27 kDa条带反应）和与抗RNP抗体相关的抗Sm抗体（12%）识别。抗SSA和抗SSB抗体罕见。