Högman C F, Eriksson L, Ring M
Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden.
Beitr Infusionsther. 1992;30:100-7.
Removal of buffy coat from the red cell preparations reduces the content of microaggregates and leukocytes and decreases transfusion side effects. However, buffy coat removal is difficult to standardize when performed manually. An automated method using a new type of plastic container system, the Optipac, and a new type of device, the Optipress, greatly improves blood component production. An initial hard centrifugation of the original whole blood provides the best results, leaving approximately 20 ml of plasma and 0.5 x 10(9) leukocytes per unit of red cells suspended in 100 ml of an additive solution (SAG-M). Improved quality and standardization of the products, reduced technician time and better working conditions for the technician are the major advantages as compared to traditional manual technique.
从红细胞制剂中去除白细胞层可降低微聚体和白细胞的含量，并减少输血副作用。然而，手动进行白细胞层去除时难以标准化。使用新型塑料容器系统Optipac和新型设备Optipress的自动化方法极大地提高了血液成分的生产效率。对原始全血进行初步的高强度离心可获得最佳效果，每单位红细胞在100 ml添加剂溶液（SAG-M）中悬浮时，会残留约20 ml血浆和0.5×10⁹白细胞。与传统手动技术相比，产品质量和标准化得到提高、技术人员时间减少以及技术人员工作条件改善是主要优势。
