Revers Frédéric, Guiraud Thomas, Houvenaghel Marie-Christine, Mauduit Thierry, Le Gall Olivier, Candresse Thierry
UMR GDPP, IBVM-INRA, BP 81, 33883 Villenave d'Ornon Cedex, France.
Mol Plant Microbe Interact. 2003 Jul;16(7):608-16. doi: 10.1094/MPMI.2003.16.7.608.
With the aim to characterize plant and viral factors involved in the molecular interactions between plants and potyviruses, a Lettuce mosaic virus (LMV)-Arabidopsis thaliana pathosystem was developed. Screening of Arabidopsis accessions with LMV isolates indicated the existence of a large variability in the outcome of the interaction, allowing the classification of Arabidopsis accessions into seven susceptibility groups. Using a reverse genetic approach, the genome-linked protein of LMV, a multifunctional protein shown to be involved in viral genome amplification and movement of potyviruses, was established as the viral determinant responsible for the ability to overcome the resistance of the Niederzenz accession to LMV-0. Preliminary genetic analyses from F2 and recombinant inbred lines available between susceptible and resistant Arabidopsis accessions revealed the existence of at least three resistance phenotypes to LMV with different genetic bases. One dominant resistance gene, designated LLM1, involved in blocking the replication or cell-to-cell movement of the LMV-0 isolate in the Columbia accession, was mapped to chromosome I and shown to be linked to the marker nga280. At the same time, genetic analyses of segregating F2 populations were consistent with the restriction of the systemic movement of the LMV-AF199 isolate in Columbia being controlled by two dominant genes and with the complete resistance to all tested LMV isolates of the Cape Verde islands (Cvi) accession being conferred by a single recessive resistance gene. Sequencing of the eukaryotic translation initiation factor 4E genes from the different LMV-resistant Arabidopsis accessions showed that these factors are not directly involved in the characterized resistance phenotypes.
为了鉴定参与植物与马铃薯Y病毒分子互作的植物和病毒因子，构建了莴苣花叶病毒（LMV）-拟南芥病理系统。用LMV分离株筛选拟南芥种质表明，互作结果存在很大差异，据此可将拟南芥种质分为7个感病组。采用反向遗传学方法，确定了LMV的基因组连接蛋白，这是一种多功能蛋白，参与病毒基因组扩增和马铃薯Y病毒的移动，是克服 Niederzenz 种质对LMV-0抗性的病毒决定因素。对易感和抗性拟南芥种质之间的F2代和重组自交系进行的初步遗传分析表明，至少存在三种对LMV具有不同遗传基础的抗性表型。一个显性抗性基因，命名为LLM1，参与阻止LMV-0分离株在哥伦比亚种质中的复制或细胞间移动，被定位到第一条染色体上，并显示与标记nga280连锁。同时，对分离的F2群体的遗传分析表明，哥伦比亚种质中LMV-AF199分离株的系统移动限制由两个显性基因控制，佛得角群岛（Cvi）种质对所有测试的LMV分离株的完全抗性由一个隐性抗性基因赋予。对不同抗LMV拟南芥种质的真核翻译起始因子4E基因进行测序表明，这些因子不直接参与所鉴定的抗性表型。
