Priya Kannian, Madhavan Hajib N, Reiser Bibiana J, Biswas Jyotrimay, Saptagirish Rambhatla, Narayana Kannan M, Rao Narsing A
Vision Research Foundation, Sankara Nethralaya, Chennai, India.
Ocul Immunol Inflamm. 2002 Dec;10(4):253-61. doi: 10.1076/ocii.10.4.253.15585.
To determine the presence of herpesvirus DNA in the aqueous humor (AH) of patients with serpiginous choroiditis using polymerase chain reaction (PCR).
AH from nine patients previously diagnosed with serpiginous choroiditis were investigated for herpes simplex virus (HSV), varicella zoster virus (VZV), and cytomegalovirus (CMV) by conventional virological methods and PCR. The PCR-positive DNA was gel-purified, extracted, and sequenced using a dye-based Applied Biosystems procedure. The sequences were processed through the National Cancer Institute's BLAST inquiry for species identification.
Culture and cytological examination of AH from all nine patients were negative for HSV, VZV, and CMV. Five were positive for VZV, one was positive for HSV, and three were wholly negative using PCR. Subsequent DNA sequencing of the positive samples authenticated the presence of VZV and HSV DNA in the respective patients.
VZV and HSV DNA were detected in a subset of patients with serpiginous choroiditis, suggesting that these viruses may function in the pathogenesis of this disease.
使用聚合酶链反应（PCR）确定匐行性脉络膜炎患者房水中疱疹病毒DNA的存在情况。
采用传统病毒学方法和PCR，对9例先前诊断为匐行性脉络膜炎患者的房水进行单纯疱疹病毒（HSV）、水痘带状疱疹病毒（VZV）和巨细胞病毒（CMV）检测。PCR阳性的DNA经凝胶纯化、提取，并使用基于染料的Applied Biosystems程序进行测序。通过美国国立癌症研究所的BLAST查询对序列进行处理以鉴定物种。
所有9例患者房水的培养和细胞学检查结果显示HSV、VZV和CMV均为阴性。PCR检测结果显示，5例VZV呈阳性，1例HSV呈阳性，3例全部为阴性。随后对阳性样本进行DNA测序，证实相应患者中存在VZV和HSV DNA。
在部分匐行性脉络膜炎患者中检测到VZV和HSV DNA，提示这些病毒可能在该疾病的发病机制中起作用。
