Sorlier Pierre, Rochas Cyrille, Morfin Isabelle, Viton Christophe, Domard Alain
Laboratoire des Matériaux Polymères et des Biomatériaux, UMR CNRS 5627, bat ISTIL, Domaine Scientifique de la Doua, 15, Bd. Latarjet, 69 622 Villeurbanne Cedex, France.
Biomacromolecules. 2003 Jul-Aug;4(4):1034-40. doi: 10.1021/bm034054n.
The use of two techniques, differential interferometry and quasi-elastic light scattering (QELS), allowed us to study solutions of chitosan varying in degree of acetylation (DA), degree of dissociation (alpha), and concentration (C(p)). With the first technique, we demonstrated the modification of the electric polarizability of the polymer chains, through a law of behavior of the variation of the refractive index increment dn/dC with DA and alpha. This brought us information on the various kinds of interactions (H-bonds, electrostatic, and hydrophobic) involved in the evolution of the solution properties. QELS experiments performed in dilute regime showed the presence of supramolecular structures depending on DA and alpha. The topology and the nature of these objects are discussed. The typical presence of aggregates and their evolution with concentration was also demonstrated in semidilute regime.
使用差分干涉测量法和准弹性光散射（QELS）这两种技术，使我们能够研究乙酰化程度（DA）、解离度（α）和浓度（C(p)）各不相同的壳聚糖溶液。通过第一种技术，我们通过折射率增量dn/dC随DA和α变化的行为规律，证明了聚合物链电极化率的改变。这为我们提供了有关溶液性质演变过程中所涉及的各种相互作用（氢键、静电和疏水相互作用）的信息。在稀溶液体系中进行的QELS实验表明，存在取决于DA和α的超分子结构。讨论了这些物体的拓扑结构和性质。在半稀溶液体系中也证明了聚集体的典型存在及其随浓度的演变。
