Strassburger D, Irlin Y, Carp H, Lurie S, Pecht M, Trainin N, Toder V
Department of Embryology, Tel-Aviv University Medical School, Israel.
Am J Reprod Immunol. 1992 Oct-Dec;28(3-4):277-80. doi: 10.1111/j.1600-0897.1992.tb00814.x.
Immunostimulation with complete Freund adjuvant (CFA) reverses the tendency to fetal loss in the CBA/J x DBA/2J mouse. First attempts to understand the mechanisms underlying this effect were to evaluate phenotypic and functional changes in the lymphocytic cell population after immunopotentiation. We demonstrated that treatment with CFA leads to diminished responses of maternal splenocytes towards paternal alloantigens and this low response cannot be improved with exogenous interleukin-2. Lymphocytes derived from spleen, para-aortic draining lymph nodes and placenta significantly suppress maternal response to paternal antigens. The effect of low fetal resorption rate is followed by marked elevation of asialo GM-1 and HNK-1-positive cells but not followed by any change of the L3T4 or Lyt-2-positive lymphocyte population in either the spleen or in draining lymph nodes. L3T4 and Lyt-2-positive cells have not been found in the placenta. An important feature was marked elevation of Mac-1-positive cells in the placentas of CFA-treated animals. The relevance of these findings to CFA-induced fetal protection is still under investigation.
用完全弗氏佐剂（CFA）进行免疫刺激可逆转CBA/J×DBA/2J小鼠的胎儿丢失倾向。最初尝试了解这种效应背后机制的方法是评估免疫增强后淋巴细胞群体的表型和功能变化。我们证明，用CFA处理会导致母体脾细胞对父本同种异体抗原的反应减弱，并且这种低反应不能通过外源性白细胞介素-2得到改善。源自脾脏、主动脉旁引流淋巴结和胎盘的淋巴细胞会显著抑制母体对父本抗原的反应。胎儿吸收率低的效应之后是去唾液酸GM-1和HNK-1阳性细胞显著升高，但脾脏或引流淋巴结中L3T4或Lyt-2阳性淋巴细胞群体没有任何变化。在胎盘中未发现L3T4和Lyt-2阳性细胞。一个重要特征是CFA处理动物的胎盘中Mac-1阳性细胞显著升高。这些发现与CFA诱导的胎儿保护的相关性仍在研究中。
