Ho H N, Chen S U, Yang Y S, Huang S C, Lee T Y, Gill T J
Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Am J Reprod Immunol. 1994 Jan;31(1):47-51. doi: 10.1111/j.1600-0897.1994.tb00846.x.
This study was designed to examine the influence of age, environment, and lymphocyte immunotherapy on fetal resorption rates in the CBA/J x DBA/2J mouse model.
After weaning, CBA/J female mice were randomly allocated to two different environments: room A was a conventional housing facility, and room B was a specific pathogen-free room. They were further divided into two groups in each room according to age (> three months and < or = three months), and mated with CBA/J, DBA/2J, or BALB/c males. The fetal resorption rates were observed. The immunization study was conducted using CBA/J females greater than three months old in room B. Preimmunization with various preparations and dosages of lymphocytes was performed 1 wk before mating with DBA/2J males, and fetal resorption rates were measured to investigate the effect of immunotherapy.
In room A, the fetal resorption rates (9.4 to 11.8%) were not significantly different among the various mating combinations using CBA/J females greater or less than three months of age. In room B, CBA/J females older than three months and mated with DBA/2J males had a higher rate of fetal resorption than those younger than three months (28.9 versus 14.2%) and had higher rate of fetal resorption than those housed and mated in room A (28.9 versus 10.9%). This fetal resorption rate was also higher than those seen with other mating combinations. Preimmunization with male BALB/c splenic lymphocytes (optimal dose 1 x 10(7) cells) was effective in decreasing fetal resorption rate (7.9%), whereas administration of normal saline, or immunization with male CBA/J or DBA/2J lymphocytes was not (20.7 to 27.0%).
Age and environment influence the spontaneous resorption rate in the CBA/J x DBA/2J mouse model. The high fetal resorption rate of older CBA/J females mated with DBA/2J males in a pathogen-free environment can be reduced by immunization with BALB/c splenic lymphocytes. These results suggested that a variety of mechanisms might initiate early pregnancy failure and that immunological modulation during implantation might be a nonspecific factor.
本研究旨在探讨年龄、环境和淋巴细胞免疫疗法对CBA/J×DBA/2J小鼠模型中胎儿吸收发生率的影响。
断奶后，将CBA/J雌性小鼠随机分配到两种不同环境中：A室为常规饲养设施，B室为无特定病原体室。根据年龄（大于3个月和小于或等于3个月）在每个房间进一步分为两组，并与CBA/J、DBA/2J或BALB/c雄性小鼠交配。观察胎儿吸收发生率。免疫研究在B室中对大于3个月龄的CBA/J雌性小鼠进行。在与DBA/2J雄性小鼠交配前1周，用不同制剂和剂量的淋巴细胞进行预免疫，并测量胎儿吸收发生率以研究免疫疗法的效果。
在A室，使用大于或小于3个月龄的CBA/J雌性小鼠进行的各种交配组合中，胎儿吸收发生率（9.4%至11.8%）无显著差异。在B室，大于3个月龄且与DBA/2J雄性小鼠交配的CBA/J雌性小鼠的胎儿吸收发生率高于小于3个月龄的小鼠（28.9%对14.2%），且高于在A室饲养和交配的小鼠（28.9%对10.9%）。这种胎儿吸收发生率也高于其他交配组合。用雄性BALB/c脾淋巴细胞（最佳剂量1×10⁷个细胞）进行预免疫可有效降低胎儿吸收发生率（7.9%），而给予生理盐水，或用雄性CBA/J或DBA/2J淋巴细胞进行免疫则无效（20.7%至27.0%）。
年龄和环境影响CBA/J×DBA/2J小鼠模型中的自然吸收发生率。在无病原体环境中，大于3个月龄的CBA/J雌性小鼠与DBA/2J雄性小鼠交配时的高胎儿吸收发生率可通过用BALB/c脾淋巴细胞进行免疫来降低。这些结果表明，多种机制可能引发早期妊娠失败，并且着床期间的免疫调节可能是一个非特异性因素。
