Toder V, Strassburger D, Irlin Y, Carp H, Pecht M, Trainin N
Department of Embryology and Teratology, Sackler School of Medicine, Tel-Aviv University, Israel.
Am J Reprod Immunol. 1990 Nov;24(3):63-6. doi: 10.1111/j.1600-0897.1990.tb01040.x.
CBA/J female mice mated with DBA/2J males show a high incidence of fetal resorptions. This paper presents data demonstrating that nonspecific immunopotentiation by complete Freund adjuvant (CFA) reversed pregnancy loss in CBA/J mothers. Immunization of more than 70 CBA/J females mated with DBA/2J males with CFA reduced the incidence of fetal resorption from 27.3 +/- 1.9 to 7.9 +/- 1.5%. The injection of Thymus Humoral Factor known to be a potent T cell stimulator did not reduce the number of fetal resorptions. The route of CFA distribution was found to be important--only foot pad injections were effective in fetal protection, whereas i.p. treatment did not reduce fetal resorptions. Fetal protection could be transferred by splenocytes of CFA-injected CBA/J mothers (9.6 +/- 5.0% fetal resorptions). Sera from treated CBA/J mice could not cause such an effect (17.6 +/- 4.6 vs. 21.3 +/- 6.1 in control animals). Thus, stimulation of the maternal immune system by nonspecific immunopotentiators can improve reproductive performance of this mouse combination which has an increased rate of pregnancy loss. Possible mechanisms of this fetal protection are discussed.
CBA/J 雌性小鼠与 DBA/2J 雄性小鼠交配时，胎儿吸收的发生率很高。本文提供的数据表明，完全弗氏佐剂（CFA）引起的非特异性免疫增强作用可逆转 CBA/J 母鼠的妊娠丢失。用 CFA 免疫 70 多只与 DBA/2J 雄性小鼠交配的 CBA/J 雌性小鼠，可使胎儿吸收的发生率从 27.3±1.9%降至 7.9±1.5%。注射已知为强力 T 细胞刺激剂的胸腺体液因子并不能减少胎儿吸收的数量。发现 CFA 的分布途径很重要——只有足垫注射对胎儿有保护作用，而腹腔注射则不能减少胎儿吸收。CFA 注射的 CBA/J 母鼠的脾细胞可传递胎儿保护作用（胎儿吸收发生率为 9.6±5.0%）。经处理的 CBA/J 小鼠的血清不会产生这种效果（与对照动物的 21.3±6.1%相比为 17.6±4.6%）。因此，非特异性免疫增强剂刺激母体免疫系统可改善这种妊娠丢失率增加的小鼠组合的生殖性能。本文讨论了这种胎儿保护的可能机制。
