Chou H, Ogawa N, Asanuma M, Hirata H, Mori A
Department of Neuroscience, Okayama University Medical School, Japan.
J Neural Transm Gen Sect. 1992;90(3):171-81. doi: 10.1007/BF01250959.
To clarify the interactions between dopamine receptors and muscarinic cholinergic receptors by which neurotransmitters may affect genetic responses, we studied the effects of the muscarinic cholinergic agonist, carbachol, and the muscarinic cholinergic antagonist, trihexyphenidyl, on levodopa-induced c-fos messenger RNA (mRNA) expression in rat striatum. Animals were administered levodopa (levodopa with one-tenth dosage of carbidopa), carbachol or thrihexyphenidyl alone or administered in combination as levodopa (100 mg/kg) + carbachol, or levodopa+trihexyphenidyl given as a single bolus. Levodopa given alone increase the expression of c-fos mRNA. Although carbachol or trihexyphenidyl alone was ineffective in inducing c-fos mRNA, the combination of levodopa and carbachol (> or = to 0.1 mg/kg) significantly suppressed the induction of c-fos mRNA as compared with levodopa given alone. The combined administration of levodopa and trihexyphenidyl showed a trend toward an additive effect on the induction of c-fos mRNA vs levodopa alone. These findings suggest that the muscarinic cholinergic system may modulate the levodopa-induced c-fos mRNA expression which then regulates the expression of other mRNAs.
为了阐明多巴胺受体与毒蕈碱型胆碱能受体之间的相互作用（神经递质可能通过这种相互作用影响基因反应），我们研究了毒蕈碱型胆碱能激动剂卡巴胆碱和毒蕈碱型胆碱能拮抗剂苯海索对左旋多巴诱导的大鼠纹状体c-fos信使核糖核酸（mRNA）表达的影响。单独给动物施用左旋多巴（左旋多巴与十分之一剂量的卡比多巴合用）、卡巴胆碱或苯海索，或联合施用左旋多巴（100毫克/千克）+卡巴胆碱，或单次推注给予左旋多巴+苯海索。单独给予左旋多巴可增加c-fos mRNA的表达。虽然单独使用卡巴胆碱或苯海索不能诱导c-fos mRNA，但与单独给予左旋多巴相比，左旋多巴和卡巴胆碱（≥0.1毫克/千克）的组合显著抑制了c-fos mRNA的诱导。左旋多巴和苯海索联合给药与单独使用左旋多巴相比，对c-fos mRNA的诱导呈现出相加效应的趋势。这些发现表明，毒蕈碱型胆碱能系统可能调节左旋多巴诱导的c-fos mRNA表达，进而调节其他mRNA的表达。
