Wong Joshua H, Balmer Yves, Cai Nick, Tanaka Charlene K, Vensel William H, Hurkman William J, Buchanan Bob B
Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA.
FEBS Lett. 2003 Jul 17;547(1-3):151-6. doi: 10.1016/s0014-5793(03)00696-3.
Application of a thiol-specific probe, monobromobimane, with proteomics and enzyme assays led to the identification of 23 thioredoxin targets in the starchy endosperm of mature wheat seeds (Triticum aestivum cv. Butte), almost all containing at least two conserved cysteines. The identified targets, 12 not known to be thioredoxin-linked, function in a spectrum of processes: metabolism (12 targets), protein storage (three), oxidative stress (three), protein degradation (two), protein assembly/folding (one) and unknown reactions (two). In addition to formulating metabolic pathways functional in the endosperm, the results suggest that thioredoxin acts in redox regulation throughout the life cycle of the seed.
将巯基特异性探针单溴代双马来酰亚胺应用于蛋白质组学和酶分析，从而鉴定出成熟小麦种子（普通小麦品种巴特）淀粉胚乳中的23个硫氧还蛋白靶标，几乎所有靶标都至少含有两个保守的半胱氨酸。所鉴定出的靶标中有12个此前未知与硫氧还蛋白相关，它们在一系列过程中发挥作用：代谢（12个靶标）、蛋白质储存（3个）、氧化应激（3个）、蛋白质降解（2个）、蛋白质组装/折叠（1个）以及未知反应（2个）。除了构建在胚乳中起作用的代谢途径外，这些结果还表明硫氧还蛋白在种子的整个生命周期中参与氧化还原调节。
