Sullivan Asa, Barkholt Lisbeth, Nord Carl Erik
Department of Laboratory Medicine, Huddinge University Hospital, Karolinska Institutet, SE-141 86 Stockholm, Sweden.
J Antimicrob Chemother. 2003 Aug;52(2):308-11. doi: 10.1093/jac/dkg346. Epub 2003 Jul 15.
The objective of this study was to compare the effect of clindamycin on the intestinal microflora in subjects ingesting yogurt with added probiotic microorganisms with the microflora in subjects ingesting placebo yogurt.
Twenty-four healthy subjects were included in the study. All subjects received 150 mg clindamycin four times daily for 7 days and 250 ml yogurt twice daily for 14 days. Faecal samples were collected before, during and after administration of clindamycin.
In the aerobic intestinal microflora, the numbers of enterococci increased after treatment in both groups, whereas other Gram-positive microorganisms decreased. In both groups, the numbers of Escherichia coli also decreased, whereas there was a concomitant increase in numbers of other Gram-negative bacilli. In the anaerobic microflora in subjects receiving yogurt with added microorganisms, the numbers of lactobacilli and bacteroides remained at the same levels throughout the study, whereas the numbers decreased in the placebo group. Other anaerobic bacteria decreased in both groups. The minimum inhibitory concentration of clindamycin against strains of bacteroides increased in both groups during the study.
The probiotic microorganisms evaluated in this study prevented ecological disturbances in the numbers of intestinal Bacteroides fragilis group species during clindamycin administration.
本研究的目的是比较克林霉素对摄入添加益生菌微生物酸奶的受试者肠道微生物群的影响与摄入安慰剂酸奶的受试者肠道微生物群的影响。
24名健康受试者纳入本研究。所有受试者每天4次服用150mg克林霉素，共7天，每天2次饮用250ml酸奶，共14天。在克林霉素给药前、给药期间和给药后采集粪便样本。
在需氧肠道微生物群中，两组治疗后肠球菌数量增加，而其他革兰氏阳性微生物数量减少。两组中大肠杆菌数量也减少，而其他革兰氏阴性杆菌数量同时增加。在摄入添加微生物酸奶的受试者的厌氧微生物群中，整个研究期间乳酸杆菌和拟杆菌数量保持在相同水平，而安慰剂组数量减少。两组中其他厌氧菌数量减少。研究期间两组中克林霉素对拟杆菌菌株的最低抑菌浓度均增加。
本研究中评估的益生菌微生物可防止克林霉素给药期间肠道脆弱拟杆菌群物种数量的生态紊乱。
