Oza A N, McKenna J P, McDowell S W J, Menzies F D, Neill S D
Department of Veterinary Science, Queen's University of Belfast, Stoney Road, Stormont, Belfast, BT4 3SD, Northern Ireland, UK.
J Antimicrob Chemother. 2003 Aug;52(2):220-3. doi: 10.1093/jac/dkg333. Epub 2003 Jul 15.
Between February 2000 and October 2001, cloacal swabs were collected from 387 broiler chicken flocks in Northern Ireland. Campylobacter isolates from the 262 positive flocks were tested with common antimicrobial agents using a disc diffusion method and by Etests. Resistance to erythromycin, gentamicin and chloramphenicol was <1%, whereas for ampicillin, nalidixic acid and tetracycline, resistance was 33%, 10% and 13%, respectively. Ciprofloxacin resistance was 3%, one of the lowest in recent reports from studies on human or poultry isolates. Sequence data of the quinolone resistance-determining region of the gyrA gene showed a mutation leading to Thr-86 to Ile substitution among highly resistant ciprofloxacin isolates. Only 0.8% of the isolates studied were resistant to four or more antibiotics.
2000年2月至2001年10月期间，从北爱尔兰的387个肉鸡群中采集泄殖腔拭子。使用纸片扩散法和Etest法，用常见抗菌剂对来自262个阳性鸡群的弯曲杆菌分离株进行检测。对红霉素、庆大霉素和氯霉素的耐药率<1%，而对氨苄西林、萘啶酸和四环素的耐药率分别为33%、10%和13%。环丙沙星耐药率为3%，是近期关于人或家禽分离株研究报告中最低的之一。gyrA基因喹诺酮耐药决定区的序列数据显示，在对环丙沙星高度耐药的分离株中存在导致苏氨酸-86突变为异亮氨酸的突变。所研究的分离株中只有0.8%对四种或更多抗生素耐药。
