Lynch Caoimhe T, Lynch Helen, Burke Sarah, Hawkins Kayleigh, Buttimer Colin, Mc Carthy Conor, Egan John, Whyte Paul, Bolton Declan, Coffey Aidan, Lucey Brigid
Department of Biological Sciences, Cork Institute of Technology, Bishopstown, T12 P928 Cork, Ireland.
NRL Campylobacter, Backweston Laboratory Complex, Young's Cross, Celbridge, W23 X3PH Kildare, Ireland.
Antibiotics (Basel). 2020 Jun 8;9(6):308. doi: 10.3390/antibiotics9060308.
Campylobacteriosis is the leading cause of human bacterial gastroenteritis, very often associated with poultry consumption. Thermophilic ( and ) isolates ( = 158) recovered from broiler neck skin and caecal contents in Ireland over a one-year period, resistant to at least one of three clinically relevant antimicrobial classes, were screened for resistance determinants. All ciprofloxacin-resistant isolates ( = 99) harboured the C257T nucleotide mutation (conferring the Thr-86-Ile substitution) in conjunction with other synonymous and nonsynonymous mutations, which may have epidemiological value. The A2075G nucleotide mutation and amino acid substitutions in L4 and L22 were detected in all erythromycin-resistant isolates ( = 5). The gene was detected in 100% ( = 119) of tetracycline-resistant isolates and three of which were found to harbour the mosaic tetracycline resistance gene . Two streptomycin-resistant isolates (isolated from the same flock) harboured , located in a multidrug resistance genomic island, containing aminoglycoside, streptothricin () and tetracycline resistance genes (truncated and mosaic ). The gene was identified in two streptomycin-resistant isolates. This study highlights the widespread acquisition of antimicrobial resistance determinants among chicken-associated isolates, through horizontal gene transfer or clonal expansion of resistant lineages. The stability of such resistance determinants is compounded by the fluidity of mobile genetic element.
弯曲杆菌病是人类细菌性肠胃炎的主要病因，常与食用家禽有关。在爱尔兰，从肉鸡颈部皮肤和盲肠内容物中分离出的嗜热弯曲杆菌菌株（n = 158），在一年时间内，对三种临床相关抗菌药物类别中的至少一种具有抗性，对其进行了耐药决定因素筛查。所有耐环丙沙星的菌株（n = 99）都携带C257T核苷酸突变（导致Thr-86-Ile替换）以及其他同义突变和非同义突变，这些突变可能具有流行病学价值。在所有耐红霉素的菌株（n = 5）中检测到A2075G核苷酸突变以及L4和L22中的氨基酸替换。在100%（n = 119）的耐四环素菌株中检测到tet（O）基因，其中三个被发现携带嵌合四环素抗性基因tet（M）。两个耐链霉素的空肠弯曲杆菌菌株（从同一鸡群中分离）携带位于多药抗性基因组岛中的aadA，该岛包含氨基糖苷、链丝菌素（sat）和四环素抗性基因（截短的tet（S）和嵌合tet（M））。在两个耐链霉素的空肠弯曲杆菌菌株中鉴定出strA基因。本研究强调了通过水平基因转移或抗性谱系的克隆扩增在鸡相关空肠弯曲杆菌菌株中广泛获得抗菌药物耐药决定因素。移动遗传元件的流动性加剧了此类耐药决定因素的稳定性。
