Owen William E, Roberts William L
ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA.
Am J Clin Pathol. 2003 Jul;120(1):121-6. doi: 10.1309/L2U6-HH5K-AYG4-8L40.
Serum and whole blood folate measurements are used to establish folate deficiency. Most methods used in clinical laboratories are automated, nonistopic methods that use folate-binding protein. Linearity, imprecision, and method comparison studies, including serum and whole blood hemolysates, were performed with the Access, Advia Centaur, ARCHITECT i2000, Elecsys 2010, and IMMULITE 2000 methods. The QuantaPhase II radioassay served as the comparison method. (Proprietary information is given in the text.) The Access and IMMULITE 2000 methods had higher systematic errors in linearity studies than the other 3 methods. The imprecision of all methods was acceptable (coefficient of variation, < 10%) even at low folate concentrations with the exception of the Elecsys 2010 (coefficient of variation, 16%). Method comparison studies using serum samples revealed calibration differences between the Access and Elecsys 2010 methods and the comparison method. Method comparison studies using whole blood samples showed poorer agreement between each of the automated methods and the comparison method than was seen with serum samples. The ARCHITECT i2000 folate assay demonstrated the best analytic performance. The poor agreement seen with whole blood hemolysates likely is due to calibration differences and differences in hemolysate preparation conditions.
血清和全血叶酸检测用于确定叶酸缺乏情况。临床实验室使用的大多数方法是自动化的、非同位素方法，这些方法使用叶酸结合蛋白。使用Access、Advia Centaur、ARCHITECT i2000、Elecsys 2010和IMMULITE 2000方法进行了线性、不精密度和方法比较研究，包括血清和全血溶血产物。QuantaPhase II放射免疫分析法作为比较方法。（文中给出了专有信息。）在线性研究中，Access和IMMULITE 2000方法的系统误差高于其他3种方法。除Elecsys 2010（变异系数为16%）外，即使在低叶酸浓度下，所有方法的不精密度均可接受（变异系数<10%）。使用血清样本的方法比较研究显示，Access和Elecsys 2010方法与比较方法之间存在校准差异。使用全血样本的方法比较研究表明，与血清样本相比，每种自动化方法与比较方法之间的一致性较差。ARCHITECT i2000叶酸检测表现出最佳的分析性能。全血溶血产物一致性较差可能是由于校准差异和溶血产物制备条件的差异。
