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荧光相关光谱实验中的后脉冲及其校正

Afterpulsing and its correction in fluorescence correlation spectroscopy experiments.

作者信息

Zhao Ming, Jin Lei, Chen Bo, Ding Yao, Ma Hui, Chen Dieyan

机构信息

Laboratory of Atomic, Molecular and Nanosciences, Tsinghua University, Beijing 100084, China.

出版信息

Appl Opt. 2003 Jul 1;42(19):4031-6. doi: 10.1364/ao.42.004031.

DOI:10.1364/ao.42.004031
PMID:12868844
Abstract

Afterpulsing arises from feedback in a photon detector. This means that each real signal pulse can be followed by an afterpulse at a later time. This effect is particularly troubling in photon correlation experiments. Few treatments of this effect have appeared in the literature, and few software programs to solve the problem have been written. We demonstrate the afterpulsing effect in fluorescence correlation spectroscopy by using different avalanche photodiodes. We prove theoretically that under simple and reasonable conditions afterpulsing in autocorrelation can be eliminated to the leading order; we have found it easy to program software for the correction. We compare our results with those from cross correlation. We also discuss some experimental parameters that may affect the afterpulsing.

摘要

后脉冲源自光子探测器中的反馈。这意味着每个真实信号脉冲之后可能会在稍后的时间出现一个后脉冲。这种效应在光子相关实验中尤其麻烦。文献中很少有关于这种效应的论述,也很少有编写解决该问题的软件程序。我们通过使用不同的雪崩光电二极管在荧光相关光谱学中展示了后脉冲效应。我们从理论上证明,在简单合理的条件下,自相关中的后脉冲可以消除到主导阶;我们发现很容易编写用于校正的软件程序。我们将我们的结果与互相关的结果进行比较。我们还讨论了一些可能影响后脉冲的实验参数。

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