Ishii Kunihiko, Tahara Tahei
Opt Express. 2015 Dec 14;23(25):32387-400. doi: 10.1364/OE.23.032387.
Afterpulsing of a photon-counting detector is a common problem in fluorescence correlation spectroscopy. We have developed a numerical procedure which eliminates the afterpulsing effect by analyzing the time reversal asymmetry of photon data that are recorded with a time-correlated single photon counting device. This method was applied to experimental data and was compared with a previous method [Rev. Sci. Instrum. 76, 033102 (2005).]. It is demonstrated that the present method can completely eliminate the afterpulsing effect even in the case of a sample solution that contains multiple fluorophores having different fluorescence lifetimes, for which the previous method underestimates the correlation amplitude. We also show a modification of the previous method incorporating the time symmetry analysis.
光子计数探测器的余辉是荧光相关光谱中常见的问题。我们开发了一种数值程序，通过分析用时间相关单光子计数装置记录的光子数据的时间反转不对称性来消除余辉效应。该方法应用于实验数据，并与先前的方法[《科学仪器评论》76, 033102 (2005)]进行了比较。结果表明，即使在样品溶液中含有多个具有不同荧光寿命的荧光团的情况下，本方法也能完全消除余辉效应，而先前的方法会低估相关幅度。我们还展示了结合时间对称性分析对先前方法的改进。
