Yates J A, Higashi G I, Lowichik A, Orihel T C, Lowrie R C, Eberhard M L
Acta Trop. 1985 Jun;42(2):157-63.
Infective stage larvae (L3) of Loa loa and Brugia malayi upon in vitro incubation with normal human serum activated the alternative complement pathway. C3 conversion products were detected on larval cuticles by eosinophil adherence and by immunofluorescence with C3c antiserum. No evidence for cuticle binding of IgG, IgA, IgM, Clq, or C4 was found by immunofluorescence. L3-induced C3 activation was inhibited by 10 mM EDTA but unaffected by 10 mM Mg++-EGTA. Human sera deficient in C2, C4, or C6 incubated with L3 resulted in C3 activation. However, sera treated with zymosan or heated for 1 h, 56 degrees C were unreactive with L3. Immunoelectrophoresis of fresh serum exposed to L3 for 1 h at 37 degrees C showed C3 cleavage products. The results indicate that these nematode L3 activate the alternative complement cascade via cuticular surface components. Larval viability was unaffected by complement activation or by adherence of eosinophils.
罗阿丝虫和马来布鲁线虫的感染期幼虫（L3）在与正常人血清进行体外孵育时，激活了替代补体途径。通过嗜酸性粒细胞黏附以及用C3c抗血清进行免疫荧光检测，在幼虫表皮上检测到了C3转化产物。通过免疫荧光未发现IgG、IgA、IgM、Clq或C4与表皮结合的证据。L3诱导的C3激活被10 mM EDTA抑制，但不受10 mM Mg++ - EGTA的影响。与L3一起孵育的缺乏C2、C4或C6的人血清导致了C3激活。然而，用酵母聚糖处理或在56℃加热1小时的血清与L3无反应。在37℃将新鲜血清与L3接触1小时后的免疫电泳显示有C3裂解产物。结果表明，这些线虫L3通过表皮表面成分激活替代补体级联反应。幼虫活力不受补体激活或嗜酸性粒细胞黏附的影响。
