Kobayashi S, Mimura Y, Notoya K, Kimura I, Kimura M
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Jpn J Pharmacol. 1992 Dec;60(4):397-401. doi: 10.1254/jjp.60.397.
Antiproliferative effects of the Japanese-Sino medicine Shimotsu-to (a combined prescription of cnidium rhizome, angelica root, peony root and rehmannia root) were investigated in the primary culture of smooth muscle cells (SMC) of mouse aorta. Fetal bovine serum (10%)-induced proliferation of primary cultured SMC was inhibited by Shimotsu-to at 4, 20, 100 or 500 micrograms/ml. The inhibitory effect was selective on SMC and due to cnidium rhizome or angelica root. The IC50 values of senkyunolide H, senkyunolide A, ligustilide and butylidenephthalide derived from cnidium were below 0.1, 1.52, 1.68 and 3.25 micrograms/ml, respectively. These results indicate that the antiproliferative effect of Shimotsu-to may depend on these cnidium-derived phthalides.
研究了汉方制剂四物汤（由川芎、当归、芍药和地黄组成的复方）对小鼠主动脉平滑肌细胞（SMC）原代培养物的抗增殖作用。四物汤在4、20、100或500微克/毫升时可抑制胎牛血清（10%）诱导的原代培养SMC增殖。这种抑制作用对SMC具有选择性，且归因于川芎或当归。川芎中提取的藁本内酯H、藁本内酯A、洋川芎内酯和丁烯基苯酞的IC50值分别低于0.1、1.52、1.68和3.25微克/毫升。这些结果表明，四物汤的抗增殖作用可能取决于这些川芎衍生的苯酞类化合物。
