Gilmore Keeta S, Srinivas Pravina, Akins Darrin R, Hatter Kenneth L, Gilmore Michael S
Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA.
Infect Immun. 2003 Aug;71(8):4759-66. doi: 10.1128/IAI.71.8.4759-4766.2003.
A model for the protracted (30-day) colonization of smooth surfaces by Streptococcus gordonii that incorporates the nutrient flux that occurs in the oral cavity was developed. This model was used to characterize the biphasic expansion of the adherent bacterial population, which corresponded with the emergence of higher-order architectures characteristic of biofilms. Biofilm formation by S. gordonii was observed to be influenced by the presence of simple sugars including sucrose, glucose, and fructose. Real-time PCR was used to quantify changes in expression of S. gordonii genes known or thought to be involved in biofilm formation. Morphological changes were accompanied by a significant shift in gene expression patterns. The majority of S. gordonii genes examined were observed to be downregulated in the biofilm phase. Genes found to be upregulated in the biofilm state were observed to encode products related to environmental sensing and signaling.
构建了一个用于描述戈登氏链球菌在光滑表面长期（30天）定殖的模型，该模型纳入了口腔中发生的营养物质流动。此模型用于表征附着细菌群体的双相扩展，这与生物膜特有的高阶结构的出现相对应。观察到蔗糖、葡萄糖和果糖等单糖的存在会影响戈登氏链球菌生物膜的形成。实时PCR用于量化已知或被认为参与生物膜形成的戈登氏链球菌基因表达的变化。形态学变化伴随着基因表达模式的显著转变。观察到所检测的大多数戈登氏链球菌基因在生物膜阶段表达下调。在生物膜状态下上调的基因被观察到编码与环境感知和信号传导相关的产物。
