Simionato M Regina, Tucker Chelsea M, Kuboniwa Masae, Lamont Gwyneth, Demuth Donald R, Tribble Gena D, Lamont Richard J
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610-0424, USA.
Infect Immun. 2006 Nov;74(11):6419-28. doi: 10.1128/IAI.00639-06. Epub 2006 Aug 21.
Porphyromonas gingivalis, one of the causative agents of adult periodontitis, develops biofilm microcolonies on substrata of Streptococcus gordonii but not on Streptococcus mutans. P. gingivalis genome microarrays were used to identify genes differentially regulated during accretion of P. gingivalis in heterotypic biofilms with S. gordonii. Thirty-three genes showed up- or downregulation by array analysis, and differential expression was confirmed by quantitative reverse transcription-PCR. The functions of the regulated genes were predominantly related to metabolism and energy production. In addition, many of the genes have no current known function. The roles of two upregulated genes, ftsH (PG0047) encoding an ATP-dependent zinc metallopeptidase and ptpA (PG1641) encoding a putative tyrosine phosphatase, were investigated further by mutational analysis. Strains with mutations in these genes developed more abundant biofilms with S. gordonii than the parental strain developed. ftsH and ptpA may thus participate in a regulatory network that constrains P. gingivalis accumulation in heterotypic biofilms. This study provided a global analysis of P. gingivalis transcriptional responses in an oral microbial community and also provided insight into the regulation of heterotypic biofilm development.
牙龈卟啉单胞菌是成人牙周炎的致病因子之一，它能在戈登链球菌的基质上形成生物膜微菌落，但不能在变形链球菌上形成。利用牙龈卟啉单胞菌基因组微阵列来鉴定在与戈登链球菌形成异型生物膜过程中牙龈卟啉单胞菌增殖期间差异表达的基因。通过阵列分析有33个基因出现上调或下调，并且通过定量逆转录 - PCR证实了差异表达。这些受调控基因的功能主要与代谢和能量产生有关。此外，许多基因目前尚无已知功能。通过突变分析进一步研究了两个上调基因的作用，这两个基因分别是编码ATP依赖性锌金属肽酶的ftsH（PG0047）和编码假定酪氨酸磷酸酶的ptpA（PG1641）。与亲本菌株相比，这些基因发生突变的菌株与戈登链球菌形成的生物膜更为丰富。因此，ftsH和ptpA可能参与了一个限制牙龈卟啉单胞菌在异型生物膜中聚集的调控网络。本研究对口腔微生物群落中牙龈卟啉单胞菌的转录反应进行了全面分析，同时也为异型生物膜发育的调控提供了见解。
