Dorey C K, Delori F C, Akeo K
Macular Disease Research Center, Eye Research Institute, Boston, MA 02114.
Curr Eye Res. 1990 Jun;9(6):549-59. doi: 10.3109/02713689008999595.
Blue light, but not green or red light, inhibited growth of retinal pigment epithelial (RPE) cells, aortic endothelial cells, and fibroblasts in vitro. Significant inhibition was observed in all 3 cell types exposed for 18 hr to blue light (425-500 nm) at 42 J/cm2. Damage was prevented by inclusion of superoxide dismutase (SOD) and catalase, providing evidence for a photooxidative mechanism. Dopa (100 microM) also caused oxidative damage that suppressed growth of all 3 cell types. A synergism of dopa and light effects was observed in endothelial cells and fibroblasts, but the agents caused additive effects on RPE cells. Endothelial cells were the most sensitive to dopa, light, and the two combined. Fibroblasts were the only cell type that exhibited greater sensitivity to light than to dopa. These data suggest that oxygen-mediated damage to the growing blood vessels in the retina of a premature infant may be exacerbated by exposure to blue light. A further implication is that restriction of RPE melanogenesis to the prenatal period of darkness and lower oxygen protects the retina from simultaneous oxidative challenge by light and by reactive species generated during oxidation of dopa released to the extracellular environment.
蓝光而非绿光或红光在体外抑制视网膜色素上皮（RPE）细胞、主动脉内皮细胞和成纤维细胞的生长。在42 J/cm²的蓝光（425 - 500 nm）照射18小时的所有3种细胞类型中均观察到显著抑制。超氧化物歧化酶（SOD）和过氧化氢酶可防止损伤，这为光氧化机制提供了证据。多巴（100 microM）也会引起氧化损伤，抑制所有3种细胞类型的生长。在内皮细胞和成纤维细胞中观察到多巴和光效应的协同作用，但这些因素对RPE细胞产生相加效应。内皮细胞对多巴、光及二者联合作用最为敏感。成纤维细胞是唯一对光比对多巴更敏感的细胞类型。这些数据表明，早产儿视网膜中正在生长的血管因暴露于蓝光而可能加剧氧介导的损伤。进一步的含义是，将RPE黑色素生成限制在产前黑暗和低氧时期可保护视网膜免受光以及释放到细胞外环境中的多巴氧化过程中产生的活性物质同时引发的氧化挑战。
