罗红霉素对鼻息肉成纤维细胞体外炎性细胞因子产生的影响。

Influence of roxithromycin on inflammatory cytokine production from nasal polyp fibroblasts in vitro.

作者信息

Suzaki Harumi, Asano Kazuhito, Yu Mei, Hisamitsu Tadashi

机构信息

Department of Otolaryngology, School of Medicine, Showa University, Tokyo, Japan.

出版信息

Acta Otolaryngol. 2003 Jun;123(5):637-42. doi: 10.1080/0001648021000028132.

DOI:10.1080/0001648021000028132

PMID:12875587

Abstract

The influence of macrolide antibiotics, roxithromycin (RXM) and josamycin (JM) on inflammatory cytokine production from human nasal polyp fibroblasts (NPFs) was examined using an in vitro cell culture technique. Addition of RXM at a concentration of 10.0 microg/ml to cell cultures suppressed both IL-6 and RANTES (but not IL-8) production in response to stimulation with 25.0 ng/ml tumor necrosis factor (TNF)-alpha. However, JM could not suppress IL-6, IL-8 and RANTES production from NPFs induced by TNF-alpha stimulation in vitro, even when added to cell cultures at a concentration of 20.0 microgram/ml. In the second part of the study, we examined the influence of RXM on cytokine mRNA expression in NPFs. Addition of RXM at a concentration of 10.0 mg/ml to cell cultures caused reduction of the mRNA expressions of both IL-6 and RANTES, which were enhanced by TNF-alpha stimulation in vitro.

摘要

采用体外细胞培养技术，研究了大环内酯类抗生素罗红霉素（RXM）和交沙霉素（JM）对人鼻息肉成纤维细胞（NPFs）炎性细胞因子产生的影响。向细胞培养物中添加浓度为10.0微克/毫升的RXM，可抑制在25.0纳克/毫升肿瘤坏死因子（TNF）-α刺激下IL-6和RANTES（但不包括IL-8）的产生。然而，即使以20.0微克/毫升的浓度添加到细胞培养物中，JM也不能抑制体外TNF-α刺激诱导的NPFs产生IL-6、IL-8和RANTES。在研究的第二部分，我们检测了RXM对NPFs中细胞因子mRNA表达的影响。向细胞培养物中添加浓度为10.0毫克/毫升的RXM，可使IL-6和RANTES的mRNA表达降低，这两种细胞因子的mRNA表达在体外被TNF-α刺激增强。