大环内酯类抗生素对鼻息肉成纤维细胞体外一氧化氮产生的抑制活性。

Suppressive activity of macrolide antibiotics on nitric oxide production from nasal polyp fibroblasts in vitro.

作者信息

Asano Kazuhito, Kamakazu Kiyoaki, Hisamitsu Tadashi, Suzaki Harumi

机构信息

Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.

出版信息

Acta Otolaryngol. 2003 Dec;123(9):1064-9. doi: 10.1080/00016480310002519.

DOI:10.1080/00016480310002519

PMID:14710909

Abstract

The influence of macrolide antibiotics on nitric oxide (NO) generation was examined using human nasal polyp fibroblasts (NPFs) in vitro. Addition of roxithromycin (RXM) at a concentration of > 7.5 microg/ml to cell cultures was shown to suppress NO production in response to stimulation with 25.0 ng/ml tumor necrosis factor (TNF)-alpha. However, jyosamycin (JM) did not suppress NO production from NPFs induced by TNF-alpha stimulation in vitro, even when added to cell cultures at a concentration of 20.0 microg/ml. We then examined the influence of RXM on inducible nitric oxide synthase (iNOS) mRNA expression in NPFs. Addition of RXM at a dose of 7.5 microg/ml to cell cultures caused reduction of iNOS mRNA expression, which was enhanced by TNF-alpha stimulation in vitro.

摘要

在体外使用人鼻息肉成纤维细胞（NPF）研究了大环内酯类抗生素对一氧化氮（NO）生成的影响。向细胞培养物中添加浓度>7.5μg/ml的罗红霉素（RXM）可抑制细胞对25.0ng/ml肿瘤坏死因子（TNF）-α刺激产生的NO。然而，即使以20.0μg/ml的浓度添加到细胞培养物中，交沙霉素（JM）在体外也不会抑制TNF-α刺激诱导的NPF产生NO。然后，我们研究了RXM对NPF中诱导型一氧化氮合酶（iNOS）mRNA表达的影响。向细胞培养物中添加7.5μg/ml剂量的RXM可导致iNOS mRNA表达降低，体外TNF-α刺激可增强这种降低。