谷胱甘肽S-转移酶M1和T1基因型与子宫内膜异位症风险：一项病例对照研究。

Glutathione S-transferase M1 and T1 genotypes and endometriosis risk: a case-controlled study.

作者信息

Lin Jun, Zhang Xinmei, Qian Yuli, Ye Yinghui, Shi Yifu, Xu Kaihong, Xu Jianyun

机构信息

Department of Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.

出版信息

Chin Med J (Engl). 2003 May;116(5):777-80.

PMID:12875700

Abstract

OBJECTIVE

To investigate the correlation between glutathione S-transferase (GST) M1 and T1 genotypes and endometriosis risk (EM).

METHODS

Polymerase chain reaction (PCR) technique was used to detect the presence or absence of the GSTM1 and GSTT1 genes in genomic DNA isolated from the blood samples of 68 Han Chinese women with endometriosis and 28 without endometriosis.

RESULTS

The frequencies of GSTM1 and GSTT1 null genotypes in women with endometriosis were 0.721 (49/68) and 0.779 (53/68), respectively, and in women without endometriosis were 0.429 (12/28) and 0.321 (9/28), respectively. There was a significant difference with regard to the frequencies of GSTM1 and GSTT1 null genotypes between the women with and without endometriosis (P < 0.01). Furthermore, the frequencies of GSTM1 and GSTT1 null genotypes were significantly higher in the patients with stage III and IV endometriosis [0.731 (38/52) and 0.788 (41/52), respectively] than in women without endometriosis (P < 0.01), and the frequency of GSTT1 null genotype was statistically higher in patients with stage I and II endometriosis [0.75 (12/16)] than in the women without endometriosis (P < 0.01). No correlation between GSTM1 and GSTT1 null genotypes and age, induced abortion or dysmenorrhea was detected in this study (P > 0.05).

CONCLUSION

GSTM1 and GSTT1 null genotypes may be risk factors for the development of endometriosis.

摘要

目的

探讨谷胱甘肽S-转移酶（GST）M1和T1基因多态性与子宫内膜异位症（EM）发病风险的相关性。

方法

采用聚合酶链反应（PCR）技术，检测68例汉族子宫内膜异位症患者及28例非子宫内膜异位症患者血样基因组DNA中GSTM1和GSTT1基因的存在情况。

结果

子宫内膜异位症患者中GSTM1和GSTT1基因缺失型频率分别为0.721（49/68）和0.779（53/68），非子宫内膜异位症患者中分别为0.429（12/28）和0.321（9/28）。子宫内膜异位症患者与非子宫内膜异位症患者GSTM1和GSTT1基因缺失型频率差异有统计学意义（P<0.01）。此外，Ⅲ、Ⅳ期子宫内膜异位症患者GSTM1和GSTT1基因缺失型频率[分别为0.731（38/52）和0.788（41/52）]显著高于非子宫内膜异位症患者（P<0.01），Ⅰ、Ⅱ期子宫内膜异位症患者GSTT1基因缺失型频率[0.75（12/16）]高于非子宫内膜异位症患者，差异有统计学意义（P<0.01）。本研究未检测到GSTM1和GSTT1基因缺失型与年龄、人工流产或痛经之间的相关性（P>0.05）。