Siouffi A M
UMR 6516, Université d'Aix Marseille, France.
J Chromatogr A. 2003 Jun 6;1000(1-2):801-18. doi: 10.1016/s0021-9673(03)00510-7.
There is a great deal of interest in continuous beds as stationary phases for both HPLC and CEC. There are various ways to prepare monoliths, by polymerization of organic species or by polymerization of silicon alkoxides. The former method has recently been reviewed, while silica based monoliths are now commercially available. The purpose of this paper is to deal with the problems associated with silica based monoliths. The most important problem is obviously the cracking and the shrinkage of the bed during drying. The second problem is monolith cladding. Much literature has been published but no definitive solution is available and thus a wide research area remains open. Monoliths are a compromise between loadability, permeability and mass transfer kinetics. Due to the better mass transfer properties of a monolithic skeleton over distinct particles, high flow rates and high speed separations are possible.
连续床作为高效液相色谱（HPLC）和毛细管电色谱（CEC）的固定相备受关注。制备整体柱有多种方法，可通过有机物种聚合或硅醇盐聚合来实现。前者方法近期已有综述，而基于二氧化硅的整体柱目前已有商业产品。本文旨在探讨与基于二氧化硅的整体柱相关的问题。最主要的问题显然是干燥过程中柱床的开裂和收缩。第二个问题是整体柱的包层。虽然已发表了大量文献，但尚无定论性的解决方案，因此仍有广阔的研究领域有待开拓。整体柱是在负载能力、渗透率和传质动力学之间的一种折衷。由于整体柱骨架相较于离散颗粒具有更好的传质性能，所以可以实现高流速和高速分离。
