Choi Jong Hee, Jang Minhee, Kim Eun-Jeong, Lee Min Jung, Park Kyoung Sun, Kim Seung-Hyun, In Jun-Gyo, Kwak Yi-Seong, Park Dae-Hun, Cho Seung-Sik, Nah Seung-Yeol, Cho Ik-Hyun, Bae Chun-Sik
Department of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
Department of Science in Korean Medicine and Brain Korea 21 Plus Program, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
J Ginseng Res. 2020 Nov;44(6):790-798. doi: 10.1016/j.jgr.2019.08.007. Epub 2019 Aug 26.
Beneficial effects of Korean Red Ginseng (KRG) on polycystic ovarian syndrome (PCOS) remains unclear.
We examined whether pretreatment (daily from 2 hours before PCOS induction) with KRG extract in water (KRGE; 75 and 150 mg/kg/day, p.o.) could exert a favorable effect in a dehydroepiandrosterone (DHEA)-induced PCOS rat model.
Pretreatment with KRGE significantly inhibited the elevation of body and ovary weights, the increase in number and size of ovarian cysts, and the elevation of serum testosterone and estradiol levels induced by DHEA. Pretreatment with KRGE also inhibited macrophage infiltration and enhanced mRNA expression levels of chemokines [interleukin (IL)-8, monocyte chemoattractant protein-1), proinflammatory cytokines (IL-1β, IL-6), and inducible nitric oxide synthase in ovaries induced by DHEA. It also prevented the reduction in mRNA expression of growth factors (epidermal growth factor, transforming growth factor-beta (EGF, TGF-β)) related to inhibition of the nuclear factor kappa-light-chain-enhancer of activated B cell pathway and stimulation of the nuclear factor erythroid-derived 2-related factor 2 pathway. Interestingly, KRGE or representative ginsenosides (Rb1, Rg1, and Rg3(s)) inhibited the activity of inflammatory enzymes cyclooxygenase-2 and iNOS, cytosolic p-IkB, and nuclear p-nuclear factor kappa-light-chain-enhancer of activated B in lipopolysaccharide-induced RAW264.7 cells, whereas they increased nuclear factor erythroid-derived 2-related factor 2 nuclear translocation.
These results provide that KRGE could prevent DHEA-induced PCOS via antiinflammatory and antioxidant activities. Thus, KRGE may be used in preventive and therapeutic strategies for PCOS-like symptoms.
韩国红参（KRG）对多囊卵巢综合征（PCOS）的有益作用尚不清楚。
我们研究了在水提取物（KRGE；75和150毫克/千克/天，口服）中用KRG提取物预处理（从诱导PCOS前2小时开始每日进行）是否能在脱氢表雄酮（DHEA）诱导的PCOS大鼠模型中发挥有利作用。
KRGE预处理显著抑制了DHEA诱导的体重和卵巢重量升高、卵巢囊肿数量和大小增加以及血清睾酮和雌二醇水平升高。KRGE预处理还抑制了巨噬细胞浸润，并增强了DHEA诱导的卵巢中趋化因子[白细胞介素（IL）-8、单核细胞趋化蛋白-1]、促炎细胞因子（IL-1β、IL-6）和诱导型一氧化氮合酶的mRNA表达水平。它还防止了与抑制活化B细胞核因子κ轻链增强子途径和刺激核因子红细胞衍生2相关因子2途径相关的生长因子（表皮生长因子、转化生长因子-β（EGF、TGF-β））mRNA表达的降低。有趣的是，KRGE或代表性人参皂苷（Rb1、Rg1和Rg3(s)）在脂多糖诱导的RAW264.7细胞中抑制了炎性酶环氧合酶-2和诱导型一氧化氮合酶的活性、胞质p-IκB以及活化B细胞核因子κ轻链增强子的核p，而它们增加了核因子红细胞衍生2相关因子2的核转位。
这些结果表明，KRGE可通过抗炎和抗氧化活性预防DHEA诱导的PCOS。因此，KRGE可用于PCOS样症状的预防和治疗策略。
