大分子拥挤效应：定性和半定量方面的成功，定量方面的挑战。
Macromolecular crowding: qualitative and semiquantitative successes, quantitative challenges.
作者信息
Hall Damien, Minton Allen P
机构信息
Section on Physical Biochemistry, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD 20892-0830, USA.
出版信息
Biochim Biophys Acta. 2003 Jul 30;1649(2):127-39. doi: 10.1016/s1570-9639(03)00167-5.
The concept of excluded volume and the theory of effects of excluded volume on the equilibria and rates of macromolecular reactions in fluid media containing high total concentrations of macromolecules ('crowded' media) are summarized. Reports of experimental studies of crowding effects published during the last year are tabulated. Limitations of current excluded volume theory are discussed, and a determination is made of conditions under which this theory may and may not be validly applied. Recently suggested novel approaches to quantitative analysis of crowding phenomena, which may help to overcome some of the limitations of current theory, are summarized.
总结了排除体积的概念以及排除体积对含有高分子总浓度高的流体介质（“拥挤”介质）中大分子反应的平衡和速率的影响理论。列出了去年发表的关于拥挤效应的实验研究报告。讨论了当前排除体积理论的局限性，并确定了该理论可以有效应用和不能有效应用的条件。总结了最近提出的用于拥挤现象定量分析的新方法，这些方法可能有助于克服当前理论的一些局限性。
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