表面等离子体共振生物传感器的现状与未来

Present and future of surface plasmon resonance biosensors.

作者信息

Homola Jirí

机构信息

Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, Prague, Czech Republic.

出版信息

Anal Bioanal Chem. 2003 Oct;377(3):528-39. doi: 10.1007/s00216-003-2101-0. Epub 2003 Jul 19.

DOI:10.1007/s00216-003-2101-0

PMID:12879189

Abstract

Surface plasmon resonance (SPR) biosensors are optical sensors exploiting special electromagnetic waves-surface plasmon-polaritons-to probe interactions between an analyte in solution and a biomolecular recognition element immobilized on the SPR sensor surface. Major application areas include detection of biological analytes and analysis of biomolecular interactions where SPR biosensors provide benefits of label-free real-time analytical technology. This paper reviews fundamentals of SPR affinity biosensors and discusses recent advances in development and applications of SPR biosensors.

摘要

表面等离子体共振（SPR）生物传感器是一种光学传感器，它利用特殊的电磁波——表面等离子体激元——来探测溶液中的分析物与固定在SPR传感器表面的生物分子识别元件之间的相互作用。主要应用领域包括生物分析物的检测和生物分子相互作用的分析，其中SPR生物传感器提供了无标记实时分析技术的优势。本文综述了SPR亲和生物传感器的基本原理，并讨论了SPR生物传感器在开发和应用方面的最新进展。