Kisidayová S, Siroka P, Lauková A
Institute of Animal Physiology, Slovak Academy of Sciences, 040 01 Kosice, Slovakia.
Folia Microbiol (Praha). 2003;48(3):408-12. doi: 10.1007/BF02931376.
The effect of nisin (in the form of Nisaplin) was determined using two species of rumen ciliate protozoa in vitro, on their co-culture bacterial population, and volatile fatty acid concentration. Nisaplin did not affect the in vitro growth of Entodinium caudatum at concentrations of 50-400 mg/L during short-term treatment (5 d). Long-term application (30 d) of Nisaplin (100 mg/L) significantly decreased growth of the Epidinium ecaudatum forma caudatum et ecaudatum but not growth of E. caudatum. Nisaplin moderately supported the growth of E. caudatum after omission of wheat gluten (source of amino acids for protozoan growth). An inhibition of Gram-positive facultative anaerobic bacterial population in the protozoan cultures (lactobacilli, enterococci, staphylococci and amylolytic streptococci) was observed during long-term Nisaplin treatment. The concentration of volatile fatty acids significantly increased during the long-term Nisaplin treatment of both cultures. The propionate concentration in the mixture of volatile fatty acids was nearly twice higher on the account of the decreased concentration (from 74 to 63%) of acetate.
利用两种瘤胃纤毛虫原生动物在体外研究了乳酸链球菌素（以Nisaplin形式）对其共培养细菌群体和挥发性脂肪酸浓度的影响。在短期处理（5天）期间，50-400mg/L浓度的Nisaplin对尾草履虫的体外生长没有影响。长期施用（30天）100mg/L的Nisaplin显著降低了尾形艾美耳球虫尾形亚种和艾美耳球虫的生长，但对尾草履虫的生长没有影响。在去除小麦面筋（原生动物生长的氨基酸来源）后，Nisaplin适度支持了尾草履虫的生长。在长期使用Nisaplin处理期间，观察到原生动物培养物中的革兰氏阳性兼性厌氧细菌群体（乳酸杆菌、肠球菌、葡萄球菌和淀粉球菌）受到抑制。在两种培养物的长期Nisaplin处理期间，挥发性脂肪酸的浓度显著增加。由于乙酸盐浓度降低（从74%降至63%），挥发性脂肪酸混合物中的丙酸盐浓度几乎高出近两倍。
