Provencio I, Loew E R, Foster R G
Department of Biology, University of Virginia, Charlottesville 22901.
Vision Res. 1992 Dec;32(12):2201-8. doi: 10.1016/0042-6989(92)90084-v.
As part of a broad study of the ocular and extraocular photoreceptors of reptiles, we have used high performance liquid chromatography (HPLC) to identify the retinoids present in whole eye extracts of the arboreal lizard Anolis carolinensis and the non-arboreal ruin lizard Podarcis sicula. Unexpectedly, only vitamin A2-derived chromophore was detected in Anolis, while a mixture of vitamin A1- and vitamin A2-derived chromophores was detected in Podarcis. These are the first examples of fully terrestrial vertebrates using vitamin A2-derived chromophore for visual pigment generation. Furthermore, microspectrophotometric (MSP) data for Anolis show a class of photoreceptor having a visual pigment with maximum absorbance at about 625 nm, some 40 nm further into the red than has been found in any terrestrial vertebrate examined to date.
作为对爬行动物眼内和眼外光感受器广泛研究的一部分，我们利用高效液相色谱法（HPLC）鉴定了树栖蜥蜴绿安乐蜥（Anolis carolinensis）和非树栖岩蜥（Podarcis sicula）全眼提取物中存在的类视黄醇。出乎意料的是，在绿安乐蜥中仅检测到维生素A2衍生的发色团，而在岩蜥中检测到维生素A1和维生素A2衍生的发色团的混合物。这些是完全陆生脊椎动物利用维生素A2衍生的发色团生成视觉色素的首个实例。此外，绿安乐蜥的显微分光光度法（MSP）数据显示，一类光感受器具有在约625 nm处具有最大吸光度的视觉色素，比迄今检查过的任何陆生脊椎动物的视觉色素在红光区域还要深入约40 nm。
