González Salvador, Astner Susi, An Wu, Goukassian David, Pathak Madhu A
Wellman Laboratories of Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
J Invest Dermatol. 2003 Aug;121(2):399-405. doi: 10.1046/j.1523-1747.2003.12355.x.
Lutein and zeaxanthin are carotenoids found in green leafy vegetables with interesting antioxidant properties. They are present in high concentrations in the fovea centralis of the human retina and their role in the prevention of age-related macula degeneration has been reported. We have investigated the effect of orally administered lutein and zeaxanthin in the cutaneous response to ultraviolet B irradiation. Female hairless SKh-1 mice receiving 0.4% and 0.04% lutein plus zeaxanthin-enriched diet for 2 wk were exposed to single doses of ultraviolet B radiation. Skin biopsies were taken at 24 and 48 h after irradiation and analyzed for the presence of apoptotic cells, proliferating cells, and expression of proliferating cell nuclear antigen. Our results show a clear ultraviolet-induced dose-dependent inflammatory response. Orally administered 0.4% lutein and zeaxanthin decreased significantly the edematous cutaneous response (p<0.01) as determined by the reduction of the UVB-induced increase of ear bifold thickening. Additionally, dietary carotenoids were efficient in reducing the ultraviolet B-induced increases in the percentage of proliferating cell nuclear antigen (p<0.05), bromodeoxyuridine (p<0.05), and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling-positive cells (p<0.01). These data demonstrate that oral supplementation of lutein and zeaxanthin diminishes the effects of ultraviolet B irradiation by reducing acute inflammatory responses and ultraviolet-induced hyperproliferative rebound.
叶黄素和玉米黄质是存在于绿叶蔬菜中的类胡萝卜素，具有有趣的抗氧化特性。它们在人类视网膜中央凹中高浓度存在，并且其在预防年龄相关性黄斑变性中的作用已有报道。我们研究了口服叶黄素和玉米黄质对皮肤紫外线B照射反应的影响。接受含0.4%和0.04%叶黄素加玉米黄质丰富饮食2周的雌性无毛SKh-1小鼠接受单剂量紫外线B辐射。在照射后24小时和48小时进行皮肤活检，分析凋亡细胞、增殖细胞的存在情况以及增殖细胞核抗原的表达。我们的结果显示出明显的紫外线诱导的剂量依赖性炎症反应。口服0.4%的叶黄素和玉米黄质显著降低了水肿性皮肤反应（p<0.01），这是通过紫外线B诱导的耳双褶增厚增加的减少来确定的。此外，膳食类胡萝卜素有效地降低了紫外线B诱导的增殖细胞核抗原百分比增加（p<0.05）、溴脱氧尿苷（p<0.05）以及末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记阳性细胞增加（p<0.01）。这些数据表明，口服补充叶黄素和玉米黄质通过减少急性炎症反应和紫外线诱导的过度增殖反弹来减轻紫外线B照射的影响。
