在膜片钳细胞中对膜染料二次谐波产生的电压敏感性进行直接测量。

Direct measurement of the voltage sensitivity of second-harmonic generation from a membrane dye in patch-clamped cells.

作者信息

Millard Andrew C, Jin Lei, Lewis Aaron, Loew Leslie M

机构信息

Center for Biomedical Imaging Technology, University of Connecticut Health Center, MC-1507, 263 Farmington Avenue, Farmington, Connecticut 06030-1507, USA.

出版信息

Opt Lett. 2003 Jul 15;28(14):1221-3. doi: 10.1364/ol.28.001221.

DOI:10.1364/ol.28.001221

PMID:12885027

Abstract

We report what is to our knowledge the first optical imaging of voltage-clamped cells by second-harmonic generation. For the membrane-staining styryl dye di-4-ANEPPS, we determined the sensitivity of second-harmonic generation to be 18%/100 mV at an excitation wavelength of 850 ns. This sensitivity is significantly better than the optimal 10%/100 mV under fluorescence and further establishes the importance of second-harmonic generation for the functional imaging of membrane potential in living cells.

摘要

据我们所知，我们首次报道了通过二次谐波产生对电压钳制细胞进行光学成像。对于膜染色苯乙烯基染料二-4-ANEPPS，我们确定在850 ns的激发波长下，二次谐波产生的灵敏度为18%/100 mV。这种灵敏度明显优于荧光下最佳的10%/100 mV，并进一步确立了二次谐波产生对于活细胞膜电位功能成像的重要性。