用非线性光学探测膜电位。
Probing membrane potential with nonlinear optics.
作者信息
Bouevitch O, Lewis A, Pinevsky I, Wuskell J P, Loew L M
机构信息
Division of Applied Physics, Hebrew University of Jerusalem, Israel.
出版信息
Biophys J. 1993 Aug;65(2):672-9. doi: 10.1016/S0006-3495(93)81126-3.
Abstract
The nonlinear optical phenomenon of second harmonic generation is shown to have intrinsic sensitivity to the voltage across a biological membrane. Our results demonstrate that this second order nonlinear optical process can be used to monitor membrane voltage with excellent signal to noise and other crucial advantages. These advantages suggest extensive use of this novel approach as an important new tool in elucidating membrane potential changes in biological systems. For this first demonstration of the effect we use a chiral styryl dye which exhibits gigantic second harmonic signals. Possible mechanisms of the voltage dependence of the second harmonic signal are discussed.
摘要
二次谐波产生的非线性光学现象被证明对生物膜上的电压具有内在敏感性。我们的结果表明，这种二阶非线性光学过程可用于监测膜电压，具有出色的信噪比和其他关键优势。这些优势表明，这种新方法作为一种重要的新工具，可广泛用于阐明生物系统中的膜电位变化。在首次展示这种效应时，我们使用了一种呈现巨大二次谐波信号的手性苯乙烯基染料。文中还讨论了二次谐波信号电压依赖性的可能机制。
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