苯乙烯基染料二次谐波产生对跨膜电位的敏感性。
Sensitivity of second harmonic generation from styryl dyes to transmembrane potential.
作者信息
Millard Andrew C, Jin Lei, Wei Mei-De, Wuskell Joseph P, Lewis Aaron, Loew Leslie M
机构信息
Department of Physiology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, Connecticut 06030-1507, USA.
出版信息
Biophys J. 2004 Feb;86(2):1169-76. doi: 10.1016/S0006-3495(04)74191-0.
Abstract
In this article we present results from the simultaneous nonlinear (second harmonic generation and two-photon excitation fluorescence) imaging and voltage clamping of living cells. Specifically, we determine the sensitivity to transmembrane potential of second harmonic generation by ANEP-chromophore styryl dyes as a function of excitation wavelength and dye structure. We have measured second harmonic sensitivities of up to 43% per 100 mV, more than a factor of four better than the nominal voltage sensitivity of the dyes under "one-photon" fluorescence. We find a dependence of voltage sensitivity on excitation wavelength that is consistent with a two-photon resonance, and there is a significant dependence of voltage sensitivity on the structure of the nonchromophore portion of the dyes.
摘要
在本文中，我们展示了对活细胞进行同步非线性（二次谐波产生和双光子激发荧光）成像及电压钳制的结果。具体而言，我们确定了由ANEP发色团苯乙烯基染料产生的二次谐波对跨膜电位的敏感度，该敏感度是激发波长和染料结构的函数。我们测量到每100 mV的二次谐波敏感度高达43%，比这些染料在“单光子”荧光下的标称电压敏感度高出四倍多。我们发现电压敏感度对激发波长的依赖性与双光子共振一致，并且电压敏感度对染料非发色团部分的结构有显著依赖性。
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