传染性支气管炎病毒免疫原性S1糖蛋白在转基因马铃薯中的表达
Expression of immunogenic S1 glycoprotein of infectious bronchitis virus in transgenic potatoes.
作者信息
Zhou Ji-Yong, Wu Jian-Xiang, Cheng Li-Qin, Zheng Xiao-Juan, Gong Hui, Shang Shao-Bin, Zhou En-Min
机构信息
Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China.
出版信息
J Virol. 2003 Aug;77(16):9090-3. doi: 10.1128/jvi.77.16.9090-9093.2003.
Abstract
The expression of infectious bronchitis virus (IBV) S1 glycoprotein in potatoes and its immunogenicity in mice and chickens were investigated. Potato plants were genetically transformed with a cDNA construct encoding the IBV S1 glycoprotein with the Agrobacterium system. Genomic DNA and mRNA analyses of the transformed plantlets confirmed the integration of the foreign cDNA into the potato genome, as well as its transcription. Mice and chickens vaccinated with the expressed IBV S1 glycoprotein produced antibodies that neutralized IBV infectivity. After three immunizations, vaccinated chickens were completely protected from virulent IBV infection. These results demonstrate that transgenic potatoes expressing IBV S1 glycoprotein can be used as a source of recombinant antigen for vaccine production.
摘要
研究了传染性支气管炎病毒（IBV）S1糖蛋白在马铃薯中的表达及其对小鼠和鸡的免疫原性。利用农杆菌系统将编码IBV S1糖蛋白的cDNA构建体导入马铃薯植株进行遗传转化。对转化后的植株进行基因组DNA和mRNA分析，证实了外源cDNA已整合到马铃薯基因组中并转录。用表达的IBV S1糖蛋白免疫的小鼠和鸡产生了中和IBV感染性的抗体。三次免疫后，接种疫苗的鸡完全免受强毒IBV感染。这些结果表明，表达IBV S1糖蛋白的转基因马铃薯可作为生产疫苗的重组抗原来源。
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8
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9
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10
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本文引用的文献
1
Plant-based vaccines: unique advantages.
Vaccine. 2001 Mar 21;19(17-19):2742-8. doi: 10.1016/s0264-410x(00)00512-0.
2
High-yield expression of a viral peptide vaccine in transgenic plants.
FEBS Lett. 2001 Jan 12;488(1-2):13-7. doi: 10.1016/s0014-5793(00)02405-4.
3
Immunization with potato plants expressing VP60 protein protects against rabbit hemorrhagic disease virus.
J Virol. 1999 May;73(5):4452-5. doi: 10.1128/JVI.73.5.4452-4455.1999.
4
Expression of immunogenic glycoprotein S polypeptides from transmissible gastroenteritis coronavirus in transgenic plants.
Virology. 1998 Sep 30;249(2):352-8. doi: 10.1006/viro.1998.9315.
5
Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene.
Vaccine. 1998 Aug;16(13):1336-43. doi: 10.1016/s0264-410x(98)80020-0.
6
Identification of amino acids involved in a serotype and neutralization specific epitope within the s1 subunit of avian infectious bronchitis virus.
Arch Virol. 1997;142(11):2249-56. doi: 10.1007/s007050050239.
7
Induction of protective immunity in chickens vaccinated with infectious bronchitis virus S1 glycoprotein expressed by a recombinant baculovirus.
J Gen Virol. 1998 Apr;79 ( Pt 4):719-23. doi: 10.1099/0022-1317-79-4-719.
8
Efficacy of a food plant-based oral cholera toxin B subunit vaccine.
Nat Biotechnol. 1998 Mar;16(3):292-7. doi: 10.1038/nbt0398-292.
9
Immunization against rabies with plant-derived antigen.
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2481-5. doi: 10.1073/pnas.95.5.2481.
10
Protective immune response to foot-and-mouth disease virus with VP1 expressed in transgenic plants.
J Virol. 1998 Feb;72(2):1688-90. doi: 10.1128/JVI.72.2.1688-1690.1998.
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