Carrillo C, Wigdorovitz A, Oliveros J C, Zamorano P I, Sadir A M, Gómez N, Salinas J, Escribano J M, Borca M V
Instituto de Virología, C.I.C.V., INTA-Castelar, Buenos Aires, Argentina.
J Virol. 1998 Feb;72(2):1688-90. doi: 10.1128/JVI.72.2.1688-1690.1998.
It has been reported recently that genes encoding antigens of bacterial and viral pathogens can be expressed in plants in a form in which they retain native immunogenic properties. The structural protein VP1 of foot-and-mouth disease virus (FMDV), which has frequently been shown to contain critical epitopes, has been expressed in different vectors and shown to induce virus-neutralizing antibodies and protection in experimental and natural hosts. Here we report the production of transformed plants (Arabidopsis thaliana) expressing VP1. Mice immunized with leaf plant extracts elicited specific antibody responses to synthetic peptides representing amino acid residues 135 to 160 of VP1, to VP1 itself, and to intact FMDV particles. Additionally, all of the immunized mice were protected against challenge with virulent FMDV. To our knowledge, this is the first study showing protection against a viral disease by immunization with an antigen expressed in a transgenic plant.
最近有报道称，编码细菌和病毒病原体抗原的基因能够在植物中以保留天然免疫原性的形式表达。口蹄疫病毒（FMDV）的结构蛋白VP1经常被证明含有关键表位，已在不同载体中表达，并在实验宿主和天然宿主中显示出能诱导病毒中和抗体并提供保护。在此，我们报告了表达VP1的转基因植物（拟南芥）的产生。用叶片植物提取物免疫的小鼠对代表VP1第135至160位氨基酸残基的合成肽、VP1本身以及完整的FMDV颗粒产生了特异性抗体反应。此外，所有免疫的小鼠都受到了保护，免受强毒性FMDV的攻击。据我们所知，这是第一项表明通过用转基因植物中表达的抗原进行免疫来预防病毒性疾病的研究。
