地笋中新克罗烷二萜类化合物的高效液相色谱分析。

HPLC analysis of neo-clerodane diterpenoids from Teucrium chamaedrys.

作者信息

Avula B, Manyam R B, Bedir E, Khan I A

机构信息

Research Institute of Pharmaceutical Sciences, National Center for Natural Products Research, The University of Mississippi, USA.

出版信息

Pharmazie. 2003 Jul;58(7):494-6.

PMID:12889535

Abstract

A simple, rapid analytical method for the quantitative determination of nine neo-clerodane diterpenoids was developed. The neo-clerodane diterpenoids present in the plant material and extracts were separated with an acetonitrile-water gradient at a flow rate of 1 mL per minute. The HPLC separation was performed on a Phenomenex Luna C18(2) (150 x 4.6 mm I.D., particle size 5 microm) reversed phase column with detection at 220 nm. The limit of detection was 0.24-0.90 microg/mL. The relative standard deviation (RSD) values for the determination of neo-clerodane diterpenoids in plant extracts were less than 3.20%. This is the first analytical method developed for qualitative and quantitative analysis of nine neo-clerodane diterpenoids by HPLC with PDA detection.

摘要

开发了一种简单、快速的定量测定9种新克罗烷二萜类化合物的分析方法。植物材料和提取物中的新克罗烷二萜类化合物以乙腈 - 水梯度在每分钟1 mL的流速下进行分离。高效液相色谱分离在Phenomenex Luna C18(2)（内径150 x 4.6 mm，粒径5微米）反相柱上进行，检测波长为220 nm。检测限为0.24 - 0.90微克/毫升。植物提取物中新克罗烷二萜类化合物测定的相对标准偏差（RSD）值小于3.20%。这是首次开发的采用高效液相色谱 - 光电二极管阵列检测法对9种新克罗烷二萜类化合物进行定性和定量分析的分析方法。