Lavigne Rob, Burkal'tseva Maria V, Robben Johan, Sykilinda Nina N, Kurochkina Lidia P, Grymonprez Barbara, Jonckx Bart, Krylov Victor N, Mesyanzhinov Vadim V, Volckaert Guido
Laboratory of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Virology. 2003 Jul 20;312(1):49-59. doi: 10.1016/s0042-6822(03)00123-5.
The complete DNA sequence of a new lytic T7-like bacteriophage phiKMV is presented. It is the first genome sequence of a member of the Podoviridae that infects Pseudomonas aeruginosa. The linear G + C-rich (62.3%) double-stranded DNA genome of 42,519 bp has direct terminal repeats of 414 bp and contains 48 open reading frames that are all transcribed from the same strand. Despite absence of homology at the DNA level, 11 of the 48 phiKMV-encoded putative proteins show sequence similarity to known T7-type phage proteins. Eighteen open reading frame products have been assigned, including an RNA polymerase, proteins involved in DNA replication, as well as structural, phage maturation, and lysis proteins. Surprisingly, the major capsid protein completely lacks sequence homology to any known protein. Also, the strong virulence toward many clinical P. aeruginosa isolates and a short replication time make phiKMV attractive for phage therapy or a potential source for antimicrobial proteins.
本文报道了一种新型裂解性T7样噬菌体phiKMV的完整DNA序列。它是感染铜绿假单胞菌的短尾噬菌体科成员的首个基因组序列。该线性双链DNA基因组富含G + C（62.3%），大小为42,519 bp，具有414 bp的直接末端重复序列，包含48个开放阅读框，均从同一条链转录。尽管在DNA水平上缺乏同源性，但48个phiKMV编码的推定蛋白中有11个与已知的T7型噬菌体蛋白具有序列相似性。已确定了18个开放阅读框产物，包括一种RNA聚合酶、参与DNA复制的蛋白以及结构、噬菌体成熟和裂解蛋白。令人惊讶的是，主要衣壳蛋白与任何已知蛋白完全缺乏序列同源性。此外，phiKMV对许多临床铜绿假单胞菌分离株具有强毒力且复制时间短，这使得它在噬菌体治疗或作为抗菌蛋白的潜在来源方面具有吸引力。
