Kim Bongjoon, Lee Jongho, Jang Jichan, Kim Jeongho, Han Hongui
Research Laboratory for Microbiology, Department of Biological Sciences, Inha University, Incheon 402-751, Republic of Korea.
Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1123-1126. doi: 10.1099/ijs.0.02463-0.
Six strains of a hitherto unknown bacterium isolated from kimchi, a fermented vegetable food produced in Korea, were characterized by using phenotypic methods, phylogenetic analysis and DNA-DNA hybridization. The novel strains were gram-positive, non-spore-forming, heterofermentative and spherical or lenticular lactic acid bacteria. Comparative 16S rRNA gene sequencing and DNA relatedness demonstrated that the unknown strains represented a novel clade within the genus Leuconostoc and were close to, but distinct from, Leuconostoc gelidum. The unknown strains were clearly distinguished from all described members of the genus Leuconostoc by using RFLP pattems of genus-specific 16S rRNA gene PCR products with a single endonuclease, BsmAI. Based on the polyphasic evidence, the unknown isolates are classified as Leuconostoc inhae sp. nov. The type strain is strain IH003T (= KCTC 3774T = DSM 15101T).
从韩国生产的发酵蔬菜食品泡菜中分离出的6株迄今未知的细菌，通过表型方法、系统发育分析和DNA-DNA杂交进行了鉴定。这些新菌株为革兰氏阳性、不形成芽孢、异型发酵的球形或透镜状乳酸菌。比较16S rRNA基因测序和DNA相关性表明，这些未知菌株代表了明串珠菌属内的一个新分支，与嗜冷明串珠菌相近，但又有所不同。通过使用单一种内切酶BsmAI对属特异性16S rRNA基因PCR产物进行RFLP分析，可将这些未知菌株与明串珠菌属的所有已描述成员清楚地区分开来。基于多相证据，这些未知分离株被分类为新种Inha明串珠菌。模式菌株为IH003T（=KCTC 3774T=DSM 15101T）。
