Vela A I, Mateos A, Collins M D, Briones V, Hutson R A, Domínguez L, Fernández-Garayzábal J F
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Int J Syst Evol Microbiol. 2003 Nov;53(Pt 6):2027-31. doi: 10.1099/ijs.0.02645-0.
Nineteen strains of Gram-positive, non-motile, non-spore-forming, catalase-positive, rod-shaped bacteria isolated from pigs were characterized by using biochemical, molecular chemical and molecular genetic methods. Two distinct groups of organisms were discerned, based on their colonial morphology, CAMP (Christie-Atkins-Munch-Petersen) reaction and numerical profile by using the API Coryne system. The first group (13 strains) gave a doubtful discrimination between Corynebacterium striatum and Corynebacterium amycolatum, whilst the second group (six strains) were identified tentatively as Corynebacterium urealyticum. Comparative 16S rRNA gene sequencing studies demonstrated that all of the isolates belonged phylogenetically to the genus CORYNEBACTERIUM: The first group of organisms was highly similar to Corynebacterium testudinoris with respect to 16S rRNA gene sequences and physiological characteristics, whereas the remaining six isolates formed a hitherto unknown subline within the genus, associated with a small subcluster of species that included Corynebacterium auriscanis and its close relatives. The unknown Corynebacterium sp. was distinguished readily from these and other species of the genus by biochemical tests. Based on both phenotypic and phylogenetic evidence, it is proposed that the new isolates from pigs should be classified as a novel species, Corynebacterium suicordis sp. nov. The type strain is P81/02(T) (=CECT 5724(T)=CCUG 46963(T)).
采用生化、分子化学和分子遗传学方法，对从猪身上分离出的19株革兰氏阳性、无运动性、不形成芽孢、过氧化氢酶阳性的杆状细菌进行了特性鉴定。根据其菌落形态、CAMP（克里斯蒂-阿特金斯-芒奇-彼得森）反应以及使用API棒状杆菌系统得出的数值图谱，可辨别出两类不同的微生物。第一组（13株）在纹带棒状杆菌和无枝菌酸棒状杆菌之间的鉴别存在疑问，而第二组（6株）初步鉴定为解脲棒状杆菌。比较16S rRNA基因测序研究表明，所有分离株在系统发育上均属于棒状杆菌属：第一组微生物在16S rRNA基因序列和生理特征方面与龟棒状杆菌高度相似，而其余6株分离株在该属内形成了一个迄今未知的亚系，与包括耳道棒状杆菌及其近缘种在内的一小类物种相关。通过生化试验，可轻易将未知的棒状杆菌属菌株与该属的其他物种区分开来。基于表型和系统发育证据，建议将从猪身上分离出的新菌株归类为一个新物种，即猪源棒状杆菌（Corynebacterium suicordis sp. nov.）。模式菌株为P81/02(T)（=CECT 5724(T)=CCUG 46963(T)）。
