Bremer F M, Rasquin F
Laboratoire d'Anatomie et Embryologie humaines, Faculté de Médecine, Université Libre de Bruxelles, Belgique.
Invest Ophthalmol Vis Sci. 1998 Nov;39(12):2466-9.
To determine the chronology of the appearance and localization of hyaluronic acid (HA) in mouse vitreous during the embryonic and early postnatal stages of development and in human vitreous during early embryonic development.
A histochemical method using the specific affinity for HA of a bovine cartilage proteoglycan was used on mouse eyes at embryonic (11 to 18 days) and early postnatal (8 and 18 days) stages. The same technique was applied to human embryonic eyes of 6, 8, 9, and 10 weeks.
In the mouse, HA is detected early (12-day embryo stage) in the equatorial vitreous and in the internal portion of the corresponding retinal epithelium, behind the anterior rim of the optic cup. Later in development, HA staining extends temporarily to the posterior vitreous and to the internal layers of the posterior retinal epithelium. In human embryos, HA staining is clearly visible in the posterior vitreous and in the equatorial vitreous, where it is more intense at all the developmental stages. From the 8-week stage onward, the internal layers of the developing retina are also heavily stained.
HA appears very early in developing vitreous of mice and humans, and staining is observed first and predominantly in the equatorial portion of the vitreous. In contrast to human embryos, HA staining in the posterior mouse fetal vitreous is only faint and transient. In both species, staining of the internal layers of the retinal epithelium is detected in the presumptive ciliary body region and in the more posterior retina. The observed temporal and regional simultaneous localization of HA staining in the vitreous and the internal layers of the retinal epithelium is suggestive of a possible role for these cells in the production of the molecule.
确定透明质酸（HA）在小鼠胚胎期和出生后早期发育阶段玻璃体中的出现时间和定位，以及在人类胚胎早期发育阶段玻璃体中的情况。
利用牛软骨蛋白聚糖对HA的特异性亲和力的组织化学方法，对胚胎期（11至18天）和出生后早期（8天和18天）的小鼠眼睛进行检测。同样的技术应用于6、8、9和10周的人类胚胎眼睛。
在小鼠中，HA在赤道部玻璃体以及相应视网膜上皮的内部被早期检测到（胚胎12天阶段），位于视杯前缘后方。在发育后期，HA染色暂时扩展到后部玻璃体和后部视网膜上皮的内层。在人类胚胎中，HA染色在后部玻璃体和赤道部玻璃体中清晰可见，在所有发育阶段此处染色更强烈。从8周阶段起，发育中的视网膜内层也被大量染色。
HA在小鼠和人类发育中的玻璃体中很早就出现，且首先在玻璃体的赤道部被观察到，染色最为明显。与人类胚胎不同，小鼠胎儿后部玻璃体中的HA染色仅微弱且短暂。在这两个物种中，视网膜上皮内层的染色在假定的睫状体区域和更靠后的视网膜中被检测到。在玻璃体和视网膜上皮内层中观察到的HA染色的时间和区域同步定位表明这些细胞在该分子产生中可能发挥作用。
