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绘制与大肠杆菌MinD分裂位点选择蛋白相互作用的MinE位点图谱。

Mapping the MinE site involved in interaction with the MinD division site selection protein of Escherichia coli.

作者信息

Ma Lu-Yan, King Glenn, Rothfield Lawrence

机构信息

Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.

出版信息

J Bacteriol. 2003 Aug;185(16):4948-55. doi: 10.1128/JB.185.16.4948-4955.2003.

DOI:10.1128/JB.185.16.4948-4955.2003
PMID:12897015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC166455/
Abstract

Interactions between the MinD and MinE proteins are required for proper placement of the Escherichia coli division septum. The site within MinE that is required for interaction with MinD was mapped by studying the effects of site-directed minE mutations on MinD-MinE interactions in yeast two-hybrid and three-hybrid experiments. This confirmed that the MinE N-terminal domain is responsible for the interaction of MinE with MinD. Mutations that interfered with the interaction defined an extended surface on one face of the alpha-helical region of the MinE N-terminal domain, consistent with the idea that the MinE-MinD interaction involves formation of a coiled-coil structure by interaction with a complementary helical surface within MinD.

摘要

MinD蛋白与MinE蛋白之间的相互作用对于大肠杆菌分裂隔膜的正确定位是必需的。通过研究定点诱变的minE突变对酵母双杂交和三杂交实验中MinD-MinE相互作用的影响，确定了MinE中与MinD相互作用所需的位点。这证实了MinE的N端结构域负责MinE与MinD的相互作用。干扰这种相互作用的突变在MinE N端结构域α螺旋区域的一个面上定义了一个延伸的表面，这与MinE-MinD相互作用涉及通过与MinD内互补螺旋表面相互作用形成卷曲螺旋结构的观点一致。

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2
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本文引用的文献

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ATP-dependent interactions between Escherichia coli Min proteins and the phospholipid membrane in vitro.体外大肠杆菌Min蛋白与磷脂膜之间的ATP依赖性相互作用。
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The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle.用于正确定位分裂位点的MinE环是一种移动结构，在大肠杆菌分裂周期中会改变其细胞内位置。
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