Zhao C R, de Boer P A, Rothfield L I
Department of Microbiology, University of Connecticut Health Center, Farmington 06030, USA.
Proc Natl Acad Sci U S A. 1995 May 9;92(10):4313-7. doi: 10.1073/pnas.92.10.4313.
The proper placement of the Escherichia coli division septum requires the MinE protein. MinE accomplishes this by imparting topological specificity to a division inhibitor coded by the minC and minD genes. As a result, the division inhibitor prevents septation at potential division sites that exist at the cell poles but permits septation at the normal division site at midcell. In this paper, we define two functions of MinE that are required for this effect and present evidence that different domains within the 88-amino acid MinE protein are responsible for each of these two functions. The first domain, responsible for the ability of MinE to counteract the activity of the MinCD division inhibitor, is located in a small region near the N terminus of the protein. The second domain, required for the topological specificity of MinE function, is located in the more distal region of the protein and affects the site specificity of placement of the division septum even when separated from the domain responsible for suppression of the activity of the division inhibitor.
大肠杆菌分裂隔膜的正确定位需要MinE蛋白。MinE通过赋予由minC和minD基因编码的分裂抑制剂拓扑特异性来实现这一点。结果，该分裂抑制剂可防止在细胞两极存在的潜在分裂位点处形成隔膜，但允许在细胞中部的正常分裂位点处形成隔膜。在本文中，我们定义了实现此效应所需的MinE的两种功能，并提供证据表明88个氨基酸的MinE蛋白内的不同结构域分别负责这两种功能。第一个结构域负责MinE抵消MinCD分裂抑制剂活性的能力，位于该蛋白N端附近的一个小区域。第二个结构域是MinE功能拓扑特异性所必需的，位于该蛋白更远端的区域，即使与负责抑制分裂抑制剂活性的结构域分离，也会影响分裂隔膜定位的位点特异性。
Zotero 插件