Jiao Kai, Salem Laura, Malone Robert
Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA.
Mol Cell Biol. 2003 Aug;23(16):5928-38. doi: 10.1128/MCB.23.16.5928-5938.2003.
Initiation of meiotic recombination in the yeast Saccharomyces cerevisiae requires at least 10 gene products. The initiation event creates double-strand breaks, which are then processed by other recombination enzymes. A variety of classical observations, such as the existence of recombination nodules, have suggested that the proteins catalyzing recombination form a complex. A variety of lines of evidence indicate that Rad50p, Mre11p, and Xrs2p interact, and genetic data suggesting interactions between Rec102p and Rec104p have been reported. It has recently been shown that Spo11p coimmunoprecipitates with Rec102p in meiosis as well. In this paper, we provide genetic and biochemical evidence that the meiosis-specific proteins Rec102p, Rec104p, and Spo11p all interact with each other in meiosis. Furthermore, we demonstrate that the interaction between Rec102p and Spo11p does not require Rec104p. Likewise, the interaction between Rec104p and Rec102p does not require Spo11p, although Spo11p may stabilize that association. The interactions suggest that Spo11p, Rec102p, and Rec104p may form a trimeric complex during the initiation of recombination.
酿酒酵母减数分裂重组的起始至少需要10种基因产物。起始事件会产生双链断裂，然后由其他重组酶进行处理。各种经典观察结果，比如重组节的存在，表明催化重组的蛋白质形成了一个复合体。多种证据表明Rad50p、Mre11p和Xrs2p相互作用，并且已有报道称遗传数据显示Rec102p和Rec104p之间存在相互作用。最近还发现，在减数分裂中Spo11p也能与Rec102p进行共免疫沉淀。在本文中，我们提供了遗传和生化证据，证明减数分裂特异性蛋白Rec102p、Rec104p和Spo11p在减数分裂过程中都相互作用。此外，我们证明Rec102p和Spo11p之间的相互作用不需要Rec104p。同样，Rec104p和Rec102p之间的相互作用也不需要Spo11p，尽管Spo11p可能会稳定这种关联。这些相互作用表明，在重组起始过程中，Spo11p、Rec102p和Rec104p可能形成一个三聚体复合体。
