Choi J B, Uchino H, Azuma K, Iwashita N, Tanaka Y, Mochizuki H, Migita M, Shimada T, Kawamori R, Watada H
Department of Medicine, Metabolism and Endocrinology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
Diabetologia. 2003 Oct;46(10):1366-74. doi: 10.1007/s00125-003-1182-9. Epub 2003 Jul 26.
AIMS/HYPOTHESIS: Bone marrow cells contain at least two distinct types of stem cells which are haematopoietic stem cells and mesenchymal stem cells. Both cells have the ability to differentiate into a variety of cell types derived from all three germ layers. Thus, bone marrow stem cells could possibly be used to generate new pancreatic beta cells for the treatment of diabetes. In this study, we investigated the feasibility of bone marrow-derived cells to differentiate into beta cells in pancreas.
Using green fluorescent protein transgenic mice as donors, the distribution of haematogenous cells in the pancreas was studied after bone marrow transplantation.
In the pancreas of green fluorescent protein chimeric mice, green fluorescent protein-positive cells were found in the islets, but none of these cells expressed insulin. Previous data has suggested that tissue injury can recruit haematopoietic stem cells or their progeny to a non-haematopietic cell fate. Therefore, low-dose streptozotocin (30 or 50 mg/kg on five consecutive days) was injected into the mice 5 weeks after bone marrow transplantation, but no green fluorescent protein-positive cells expressing insulin were seen in the islets or around the ducts of the pancreas.
CONCLUSIONS/INTERPRETATION: Our data suggests that bone marrow-derived cells are a distinct cell population from islet cells and that transdifferentiation from bone marrow-derived cells to pancreatic beta cells is rarely observed.
目的/假设：骨髓细胞包含至少两种不同类型的干细胞，即造血干细胞和间充质干细胞。这两种细胞都有能力分化为源自所有三个胚层的多种细胞类型。因此，骨髓干细胞有可能用于生成新的胰岛β细胞以治疗糖尿病。在本研究中，我们调查了骨髓来源的细胞在胰腺中分化为β细胞的可行性。
以绿色荧光蛋白转基因小鼠作为供体，在骨髓移植后研究造血细胞在胰腺中的分布。
在绿色荧光蛋白嵌合小鼠的胰腺中，胰岛中发现了绿色荧光蛋白阳性细胞，但这些细胞均不表达胰岛素。先前的数据表明，组织损伤可促使造血干细胞或其后代转向非造血细胞命运。因此，在骨髓移植5周后给小鼠注射低剂量链脲佐菌素（连续5天，30或50mg/kg），但在胰岛或胰腺导管周围未见表达胰岛素的绿色荧光蛋白阳性细胞。
结论/解读：我们的数据表明，骨髓来源的细胞是与胰岛细胞不同的细胞群体，并且很少观察到骨髓来源的细胞向胰腺β细胞的转分化。
