检测棉塞和月经成分上的金黄色葡萄球菌生物膜。
Detection of Staphylococcus aureus biofilm on tampons and menses components.
作者信息
Veeh Richard H, Shirtliff Mark E, Petik Jill R, Flood Janine A, Davis Catherine C, Seymour Jon L, Hansmann Melanie A, Kerr Kathy M, Pasmore Mark E, Costerton John W
机构信息
Center for Biofilm Engineering, Montana State University, Bozeman, Montana 59717, USA.
出版信息
J Infect Dis. 2003 Aug 15;188(4):519-30. doi: 10.1086/377001. Epub 2003 Jul 25.
Culturing has detected vaginal Staphylococcus aureus in 10%-20% of women. Because growth mode can affect virulence expression, this study examined S. aureus-biofilm occurrence in 44 paired-tampon and vaginal-wash-specimens from 18 prescreened women, using fluorescent in situ hybridization (FISH). All 44 specimens were also analyzed for S. aureus by standard culturing on mannitol salt agar, which produced positive results for 15 of the 44 specimens. FISH detected S. aureus cells in all 44 specimens, and S. aureus biofilm was observed in 37 of the 44 specimens. Independent confirmation of the presence of S. aureus in specimens from all 18 women was also obtained by amplification, via polymerase chain reaction, of an S. aureus-specific nuclease gene. The results of this study demonstrate that S. aureus biofilm can form on tampons and menses components in vivo. Additionally, the prevalence of vaginal S. aureus carriage may be more prevalent than what is currently demonstrated by standard culturing techniques.
培养检测发现，10%至20%的女性阴道中存在金黄色葡萄球菌。由于生长模式会影响毒力表达，本研究采用荧光原位杂交（FISH）技术，对18名预先筛选的女性的44对棉塞和阴道冲洗样本中的金黄色葡萄球菌生物膜形成情况进行了检测。所有44个样本还通过在甘露醇盐琼脂上进行标准培养来分析金黄色葡萄球菌，44个样本中有15个产生了阳性结果。FISH在所有44个样本中均检测到了金黄色葡萄球菌细胞，并且在44个样本中的37个中观察到了金黄色葡萄球菌生物膜。通过聚合酶链反应扩增金黄色葡萄球菌特异性核酸酶基因，也独立证实了所有18名女性样本中均存在金黄色葡萄球菌。本研究结果表明，金黄色葡萄球菌生物膜可在体内的棉塞和月经成分上形成。此外，阴道金黄色葡萄球菌携带率可能比目前标准培养技术所显示的更为普遍。
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